Department of Veterinary Medicine, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India.
Department of Veterinary Medicine, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Rampura Phul, 151103, Punjab, India.
Sci Rep. 2024 Sep 20;14(1):21975. doi: 10.1038/s41598-024-72572-z.
The current study aimed at population genetic characterization of B. vogeli based on the cytochrome b (cyt b) gene sequences (≥ 685 bp) available in the GenBank. Phylogenetic trees placed all the sequences of B. vogeli in a single large monophyletic clade; however, it was further divided into two subclades (Bv1 and Bv2). Out of seven nucleotide variations observed between Bv1 and Bv2 subclades, four were synonymous (G92A, C170T, T488C and A659G), and three were non-synonymous (G324A, C438A and G465A) resulting in amino acid substitutions at three places (V108I, L146I and V155I). Within different B. vogeli populations, the nucleotide and haplotype diversities were low. The median-joining haplotype network revealed only two haplotypes (Hap_1 and Hap_2). A geographical sub-structuring was noticed in the B. vogeli populations, with moderate genetic differentiation (F = 0.05000; P < 0.05) and a very high gene flow (Nm = 4.75) between Indian and Chinese populations. Neutrality tests and mismatch distributions for the Indian population and the overall dataset of B. vogeli indicated a constant population size. This study provides the first insight into the genetic characterization, population genetics and haplotype network of B. vogeli based on the cyt b gene.
本研究旨在基于已在 GenBank 中可用的细胞色素 b (cyt b) 基因序列（≥685 bp）对 B. vogeli 进行群体遗传特征分析。系统发育树将所有 B. vogeli 序列置于一个单一的大型单系分支中；然而，它进一步分为两个亚分支 (Bv1 和 Bv2)。在 Bv1 和 Bv2 亚分支之间观察到的七个核苷酸变异中，有四个是同义的 (G92A、C170T、T488C 和 A659G)，三个是非同义的 (G324A、C438A 和 G465A)，导致三个位置的氨基酸替换 (V108I、L146I 和 V155I)。在不同的 B. vogeli 种群中，核苷酸和单倍型多样性较低。中位数连接单倍型网络仅显示了两种单倍型 (Hap_1 和 Hap_2)。在 B. vogeli 种群中注意到了地理亚结构，印度和中国种群之间存在中度遗传分化 (F=0.05000；P<0.05) 和非常高的基因流 (Nm=4.75)。印度种群和 B. vogeli 总体数据集的中性检验和不匹配分布表明种群大小保持恒定。本研究首次基于 cyt b 基因对 B. vogeli 的遗传特征、群体遗传学和单倍型网络进行了分析。
