Behera Bijay Kumar, Baisvar Vishwamitra Singh, Kunal Swaraj Priyaranjan, Meena Dharmendra Kumar, Panda Debarata, Pakrashi Sudip, Paria Prasenjit, Das Pronob, Bhakta Dibakar, Debnath Dipesh, Roy Suvra, Suresh V R, Jena J K
a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
c ICAR-Central Coastal Agricultural Research Institute , Old Goa , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Mar;29(2):199-205. doi: 10.1080/24701394.2016.1267156. Epub 2016 Dec 26.
The population structure and genetic diversity of Rohu (Labeo rohita Hamilton, 1822) was studied by analysis of the partial sequences of mitochondrial DNA cytochrome b region. We examined 133 samples collected from six locations in three geographically isolated rivers of India. Analysis of 11 haplotypes showed low haplotype diversity (0.00150), nucleotide diversity (π) (0.02884) and low heterogeneity value (0.00374). Analysis of molecular variance (AMOVA) revealed the genetic diversity of L. rohita within population is very high than between the populations. The Fst scores (-0.07479 to 0.07022) were the indication of low genetic structure of L. rohita populations of three rivers of India. Conspicuously, Farakka-Bharuch population pair Fst score of 0.0000, although the sampling sites are from different rivers. The phylogenetic reconstruction of unique haplotypes revealed sharing of a single central haplotype (Hap_1) by all the six populations with a point mutations ranging from 1-25 nucleotides.
通过分析线粒体DNA细胞色素b区域的部分序列，研究了印度鲤（Labeo rohita Hamilton，1822）的种群结构和遗传多样性。我们检测了从印度三条地理隔离河流的六个地点采集的133个样本。对11个单倍型的分析显示单倍型多样性较低（0.00150）、核苷酸多样性（π）（0.02884）以及低异质性值（0.00374）。分子方差分析（AMOVA）表明，印度鲤种群内的遗传多样性远高于种群间。Fst值（-0.07479至0.07022）表明印度三条河流的印度鲤种群遗传结构较低。值得注意的是，法拉卡-巴鲁赫种群对的Fst值为0.0000，尽管采样地点来自不同河流。独特单倍型的系统发育重建显示，所有六个种群共享一个单一的中心单倍型（Hap_1），其点突变范围为1至25个核苷酸。
