Parasitology Laboratory, Zoology and Entomology Department, Faculty of Science, Assiut University, Assiut, 71526, Egypt.
Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, 52571, Buraydah, Saudi Arabia.
BMC Vet Res. 2024 Sep 20;20(1):419. doi: 10.1186/s12917-024-04241-2.
This study aimed to investigate the antiparasitic and anti-inflammatory potential of pumpkin seed oil in mice infected with Trichinella spiralis by demonstrating its impact on MMP-9 expression and pathogenesis during the intestinal and muscular phases.
In this study, 100 mice were divided into five groups: an infected group, a pumpkin seed oil-treated group (1.5 mg/kg BW, administered three times per week), an albendazole-treated group, a native control group, and a pumpkin oil control group. Gas chromatography-mass spectrometry analysis of the pumpkin seed oil revealed a broad spectrum of biologically active compounds. The pumpkin seed oil treatment led to a significant reduction in the parasite burden, with a 75% decrease in adult worms and a 66% decrease in encysted larvae. Additionally, the infected animals treated with pumpkin oil exhibited a marked reduction in intestinal inflammation, characterized by a progressive increase in goblet cells. The number of encysted larvae in the diaphragm and muscle tissues was also significantly decreased. Furthermore, pumpkin seed oil treatment significantly reduced MMP-9 levels in both intestinal and muscular tissues, highlighting its potential to attenuate inflammation.
These findings underscore the effectiveness of pumpkin seed oil as anti-inflammatory and antiparasitic agent.
本研究旨在通过观察基质金属蛋白酶-9（MMP-9）表达的变化，探讨南瓜籽油对感染旋毛虫小鼠的抗寄生虫和抗炎作用，研究南瓜籽油在肠道和肌肉期对旋毛虫感染的影响。
100 只小鼠随机分为五组：感染组、南瓜籽油治疗组（1.5mg/kgBW，每周三次）、阿苯达唑治疗组、天然对照组和南瓜油对照组。气相色谱-质谱分析显示南瓜籽油含有多种生物活性化合物。南瓜籽油治疗组寄生虫负荷显著降低，成虫减少 75%，囊包幼虫减少 66%。此外，南瓜籽油治疗的感染动物肠道炎症明显减轻，杯状细胞逐渐增多。膈肌和肌肉组织中的囊包幼虫数量也显著减少。此外，南瓜籽油治疗组可显著降低肠道和肌肉组织中 MMP-9 水平，表明其具有抗炎作用。
这些发现强调了南瓜籽油作为一种抗炎和抗寄生虫药物的有效性。
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