Department of Parasitology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
Department of Medical Parasitology, Faculty of Medicine 2, Assiut University, Assiut, Egypt.
Ann Parasitol. 2021;67(4):591-602. doi: 10.17420/ap6704.375.
Trichinellosis is a serious worldwide parasitic zoonosis. The available therapy for the treatment of Trichinella spiralis is not satisfactory. Therefore, the recovery of effective treatment is required. This work aimed at evaluating of the in vitro effect of silver nanoparticles (AgNPs) on muscle larvae of Trichinella. The present study investigated the larvicidal properties of chemical and myrrh AgNPs on muscle larvae (ML) of T. spiralis. The used AgNPs were chemically prepared using NaBH4 as reducing agent and biosynthesized using methanolic myrrh extract. Characterization of synthesized AgNPs was monitored via UV-vis spectrophotometry, Fourier transform infrared spectroscopy and transmission electron microscopy (TEM) studies. The ML incubated with AgNPs at concentrations ranged from 1 μg/ml to 20 μg/ml. Chemical and biosynthesized AgNPs revealed marked larvicidal effect against ML of Trichinella. Additionally, this in vitro study showed degenerative changes affecting the cuticle of AgNPs treated ML. The effectiveness of AgNPs on the infectivity of Trichinella ML was also assessed. The results showed complete inhibition of the infectivity of ML exposed to sublethal doses of chemical and myrrh prepared AgNPs when used to infect animal models. This is the first report where myrrh synthesized AgNPs have been tested for their anthelminthic activity against Trichinella in an in vitro model.
旋毛虫病是一种严重的全球性寄生虫病。目前治疗旋毛虫病的方法并不令人满意，因此需要寻找更有效的治疗方法。本研究旨在评估银纳米粒子（AgNPs）对旋毛虫肌肉幼虫的体外疗效。本研究调查了化学法和没药 AgNPs 对旋毛虫肌肉幼虫（ML）的杀幼虫特性。所使用的 AgNPs 是通过使用 NaBH4 作为还原剂化学制备的，并且是通过甲醇没药提取物生物合成的。通过紫外-可见分光光度法、傅里叶变换红外光谱和透射电子显微镜（TEM）研究监测合成 AgNPs 的特性。将 ML 与浓度范围为 1μg/ml 至 20μg/ml 的 AgNPs 孵育。化学法和生物合成 AgNPs 对旋毛虫 ML 均显示出明显的杀幼虫作用。此外，这项体外研究显示，AgNPs 处理的 ML 的角质层受到退行性变化的影响。还评估了 AgNPs 对旋毛虫 ML 感染力的影响。结果表明，当用于感染动物模型时，暴露于亚致死剂量的化学法和没药制备的 AgNPs 的 ML 的感染力完全受到抑制。这是首次在体外模型中测试没药合成的 AgNPs 对旋毛虫的驱虫活性的报告。
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