Department of Medical Pharmacology, Faculty of Medicine, Assiut University, Assiut 71526, Egypt.
Department of Pharmacology and Toxicology, Unaizah College of Pharmacy, Qassim University, Buraidah 51452, Saudi Arabia.
Molecules. 2022 Aug 31;27(17):5598. doi: 10.3390/molecules27175598.
The main bioactive constituents in the standardized leaf extract (EGb 761) are the terpene lactones and flavonoid glycosides. EGb 761's antioxidant and anti-inflammatory properties have previously been demonstrated. Indomethacin-induced gastric ulcers have a multifactorial etiology and represent a major restriction to its therapeutic utility. The underlying ulcerogenic process involves oxidative and inflammatory biomolecular insults. This study was performed to explore the curative and preventative benefits of EGb 761 in experimentally-induced ulcers. To develop gastric ulcers in mice, indomethacin (40 mg/kg) was administered orally. EGb 761 (200 mg/kg) was given by gavage for 7 days before (preventative) and after (therapeutic) indomethacin administration. The histological alterations and macroscopic mucosal lesions were assessed. In gastric tissue homogenates, malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), and inflammatory cytokines were measured. The expressions of cyclooxygenase-2 (COX-2), cytokines, and proliferating cell nuclear antigen (PCNA) in the stomach mucosa were also investigated. The ulcer index, histological alterations, gastric oxidants, and inflammatory biomarkers were all significantly increased by indomethacin. In stomach specimens, it increased COX-2 and PCNA expression. EGb 761 treatments, both prophylactic and therapeutic, resulted in significant reductions in ulcer lesions, nitrosative and oxidative damage, and inflammatory markers, along with the lowering of COX-2 and PCNA expressions. Furthermore, in the fight against stomach ulcers, EGb 761 treatment was found to be more efficient than prevention.
标准化叶提取物（银杏叶提取物 761，EGb 761）中的主要生物活性成分是萜烯内酯和类黄酮糖苷。EGb 761 的抗氧化和抗炎特性以前已经得到证实。吲哚美辛诱导的胃溃疡具有多因素病因，这是其治疗应用的主要限制。潜在的溃疡形成过程涉及氧化和炎症生物分子损伤。这项研究旨在探索 EGb 761 在实验性溃疡中的治疗和预防作用。为了在小鼠中诱导胃溃疡，给予吲哚美辛（40mg/kg）口服。在给予吲哚美辛之前（预防性）和之后（治疗性），通过灌胃给予 EGb 761（200mg/kg）共 7 天。评估了组织学改变和宏观黏膜损伤。在胃组织匀浆中，测量丙二醛（MDA）、还原型谷胱甘肽（GSH）、一氧化氮（NO）和炎症细胞因子。还研究了胃黏膜中环氧化酶-2（COX-2）、细胞因子和增殖细胞核抗原（PCNA）的表达。吲哚美辛显著增加了溃疡指数、组织学改变、胃氧化剂和炎症生物标志物。在胃标本中，它增加了 COX-2 和 PCNA 的表达。预防性和治疗性的 EGb 761 治疗均导致溃疡病变、硝化和氧化损伤以及炎症标志物的显著减少，同时降低了 COX-2 和 PCNA 的表达。此外，在对抗胃溃疡方面，EGb 761 治疗比预防更有效。
