Assessment of pesticide residues: a comprehensive analysis of seasonal trends and health implications.

This study assessed the presence of eight pesticide residues in the Indus River, Mianwali, Pakistan, focusing on three sampling sites (S1, S2, and S3) in water, sediment, and the fish species during both dry and wet seasons. Analysis was conducted using gas chromatography with an electron capture detector. Results indicated elevated pesticide concentrations in both seasons, with levels of 0.84 and 0.62 μg/L in water, 12.47 and 9.21 μg/g/dw in sediment, and 17.33 and 12.17 μg/g/ww in fish, with higher concentrations observed during the dry season. Cypermethrin and carbofuran were the primary pesticides detected in water, while endosulfan and cypermethrin were dominant in sediment and fish tissue, often exceeding standard safety thresholds. Principal Component Analysis (PCA) and cluster analysis revealed stronger correlations between sediment and fish muscle, with varying associations among pesticides across seasons. The Hazard Index (HI) surpassed 1 in both seasons, signaling potential health risks to humans. These findings underscore the substantial risk agricultural pesticides pose to the aquatic ecosystem and food chain, highlighting the urgent need for sustainable agricultural practices and stricter regulations to minimize pesticide use and encourage eco-friendly pest management strategies.