La Xiaoqin, Zhang Zhaoyan, Liang Jingyi, Li Hanqing, Pang Yan, He Xiaoting, Kang Yurui, Wu Changxin, Li Zhuoyu
Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China.
Shanxi Provincial Key Laboratory of Medical Molecular Cell Biology, Shanxi University, Taiyuan, China.
J Sci Food Agric. 2025 Jan 30;105(2):1330-1342. doi: 10.1002/jsfa.13923. Epub 2024 Sep 21.
Nonalcoholic fatty liver disease (NAFLD), a chronic metabolic disorder marked by excessive lipid deposition, represents a considerable health burden with no established efficacious treatment strategy. Quinoa (Chenopodium quinoa Willd.), valued for its health benefits, is replete with flavonoid bioactives. The aims of this work are to isolate and purify flavonoids from quinoa whole grain that can intervene in NAFLD and to elucidate some of the underlying mechanisms.
Chenopodium quinoa Willd. flavonoids (CQWF) were obtained successfully through an optimized ultrasonic extraction methodology, followed by isolation and purification utilizing macroporous resin D101. The study then explored the therapeutic potential of CQWF and its eluted fractions in models emulating NAFLD conditions: an in vitro fatty liver cell model induced by oleic acid (OA) and palmitic acid (PA) in the HepG2 and BEL-7402 cell lines, and an in vivo high-fat diet (HFD)-induced NAFLD model in C57BL/6N mice. The findings revealed a comprehensive mitigating effect of CQWF30 on NAFLD, manifesting in reduced intracellular lipid accumulation in steatotic hepatocytes and a concerted downregulation of key lipid metabolism genes, CD36 and FASN. Administration of CQWF30 reduced triglyceride (TG) levels in both the cellular model and the livers of HFD-fed mice. It also reduced serum concentrations of TG, total cholesterol (T-CHO), low-density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), and alanine aminotransferase (ALT), while increasing high-density lipoprotein cholesterol (HDL-C) in the mice.
These results highlighted the promising therapeutic capacity of CQWF, particularly CQWF30. This research advances the exploration and utilization of flavonoids derived from quinoa whole grain, providing innovative dietary intervention strategies for NAFLD. © 2024 Society of Chemical Industry.
非酒精性脂肪性肝病（NAFLD）是一种以脂质过度沉积为特征的慢性代谢紊乱疾病，是一个相当大的健康负担，目前尚无成熟有效的治疗策略。藜麦（Chenopodium quinoa Willd.）因其对健康有益而受到重视，富含类黄酮生物活性成分。本研究的目的是从藜麦全谷物中分离和纯化可干预NAFLD的类黄酮，并阐明一些潜在机制。
通过优化的超声提取方法成功获得了藜麦（Chenopodium quinoa Willd.）类黄酮（CQWF），随后利用大孔树脂D101进行分离和纯化。该研究随后在模拟NAFLD条件的模型中探索了CQWF及其洗脱组分的治疗潜力：在HepG2和BEL-7402细胞系中由油酸（OA）和棕榈酸（PA）诱导的体外脂肪肝细胞模型，以及在C57BL/6N小鼠中由高脂饮食（HFD）诱导的体内NAFLD模型。研究结果显示CQWF30对NAFLD具有全面的缓解作用，表现为脂肪变性肝细胞内脂质积累减少以及关键脂质代谢基因CD36和FASN的协同下调。给予CQWF30可降低细胞模型和高脂饮食喂养小鼠肝脏中的甘油三酯（TG）水平。它还降低了小鼠血清中TG、总胆固醇（T-CHO）、低密度脂蛋白胆固醇（LDL-C）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）的浓度，同时提高了高密度脂蛋白胆固醇（HDL-C）。
这些结果突出了CQWF，特别是CQWF30的良好治疗潜力。本研究推进了对藜麦全谷物中类黄酮的探索和利用，为NAFLD提供了创新的饮食干预策略。© 2024化学工业协会。
