La Xiaoqin, Zhang Zhaoyan, Dong Cunli, Li Hanqing, He Xiaoting, Kang Yurui, Wu Changxin, Li Zhuoyu
Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, China.
School of Life Science, Shanxi University, Taiyuan 030006, China.
Foods. 2024 Sep 26;13(19):3076. doi: 10.3390/foods13193076.
Non-alcoholic fatty liver disease (NAFLD) is a severe hepatic health threat with no effective treatment. Based on the results that Chenopodium quinoa Willd. flavonoids eluted with 30% ethanol (CQWF30) can effectively alleviate NAFLD, this study employed ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) to analyze the components of CQWF30., and screened for flavonoids with potential NAFLD-mitigating effects through network pharmacology. In vitro models using HepG2 and BEL-7402 cell lines induced with free fatty acid (FFA) showed that isorhamnetin administration reduced intracellular lipid deposition and reversed elevated triglyceride (TG) and total cholesterol (T-CHO) levels. In vivo experiments in high-fat diet (HFD) mice demonstrated that isorhamnetin significantly lowered serum and liver fat content, mitigated liver damage, and modulated bile acid metabolism by upregulating FXR and BSEP and downregulating SLCO1B3. Consequently, isorhamnetin shows promise as a treatment for NAFLD due to its lipid-lowering and hepatoprotective activities.
非酒精性脂肪性肝病（NAFLD）是一种严重威胁肝脏健康且尚无有效治疗方法的疾病。基于藜麦黄酮类化合物用30%乙醇洗脱物（CQWF30）能有效缓解NAFLD的结果，本研究采用超高效液相色谱-电喷雾电离-串联质谱法（UPLC-ESI-MS/MS）分析CQWF30的成分，并通过网络药理学筛选具有潜在缓解NAFLD作用的黄酮类化合物。使用游离脂肪酸（FFA）诱导的HepG2和BEL-7402细胞系的体外模型表明，给予异鼠李素可减少细胞内脂质沉积，并逆转甘油三酯（TG）和总胆固醇（T-CHO）水平的升高。在高脂饮食（HFD）小鼠中进行的体内实验表明，异鼠李素显著降低血清和肝脏脂肪含量，减轻肝脏损伤，并通过上调法尼酯X受体（FXR）和胆汁盐输出泵（BSEP）以及下调有机阴离子转运多肽1B3（SLCO1B3）来调节胆汁酸代谢。因此，异鼠李素因其降脂和肝脏保护活性而有望成为治疗NAFLD的药物。
