Chen Shaohua, Zhou Xuefeng, Wang Xinyue, Li Heng, An Rong, Qian Yunzhu
School of Materials Science and Engineering/Herbert Gleiter Institute of Nanoscience, Nanjing University of Science and Technology, Nanjing 210094, China.
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114245. doi: 10.1016/j.colsurfb.2024.114245. Epub 2024 Sep 13.
Excessive reactive oxygen species (ROS) and bacterial infection significantly disrupt the microenvironment of tissue regeneration. Magnetic nanoparticles in combination with magnetic fields are emerging strategies to regulate tissue regeneration. In this work, ZnFeO (ZFO) nanoparticles were prepared and subsequently decorated with polydopamine (PDA) and RGD peptide. The modified ZFO nanoparticles were loaded into polylactic-glycolic acid/polycaprolactone (PLGA/PCL) scaffolds as PP-ZFO/PDA-RGD magnetic scaffolds. Physicochemical, anti-ROS, antibacterial and osteogenic properties were evaluated in vitro. Under 30 mT static magnetic field, the PP-ZFO/PDA-RGD scaffold significantly enhanced the expression of ALP, RUNX2, BMP2 and mineralized nodules. It indicated that the magnetic system could promote the attachment, proliferation, differentiation and mineralization of MC3T3-E1 cells. The scaffold showed excellent ROS scavenging ability via the additive effect of ZFO, PDA and RGD. The remarkable antibacterial properties of Zn²⁺ and Fe³⁺ endowed the scaffolds with excellent antibacterial activity against E. coli and S. aureus. These results demonstrate that the PP-ZFO/PDA-RGD based magnetic system is a promising approach for bone regeneration under complex microenvironment.
过量的活性氧（ROS）和细菌感染会显著破坏组织再生的微环境。磁性纳米颗粒与磁场相结合是调节组织再生的新兴策略。在这项工作中，制备了ZnFeO（ZFO）纳米颗粒，随后用聚多巴胺（PDA）和RGD肽进行修饰。将修饰后的ZFO纳米颗粒负载到聚乳酸-乙醇酸/聚己内酯（PLGA/PCL）支架中，制成PP-ZFO/PDA-RGD磁性支架。对其理化性质、抗ROS性能、抗菌性能和成骨性能进行了体外评估。在30 mT静磁场下，PP-ZFO/PDA-RGD支架显著增强了碱性磷酸酶（ALP）、Runx2、骨形态发生蛋白2（BMP2）的表达以及矿化结节的形成。这表明该磁性系统可促进MC3T3-E1细胞的黏附、增殖、分化和矿化。通过ZFO、PDA和RGD的协同作用，该支架表现出优异的ROS清除能力。Zn²⁺和Fe³⁺显著的抗菌性能赋予了支架对大肠杆菌和金黄色葡萄球菌优异的抗菌活性。这些结果表明，基于PP-ZFO/PDA-RGD的磁性系统是在复杂微环境下促进骨再生的一种有前景的方法。
