Abdulfattah Shaimaa Y, Salman Alagely Huda, Abid Kathum Omer, Samawi Farah T
Medical and Molecular Department, Biotechnology Research Center, Al-Nahrain University, Jadriya, Baghdad, Iraq.
Medical and Molecular Department, Biotechnology Research Center, Al-Nahrain University, Jadriya, Baghdad, Iraq.
Hum Immunol. 2024 Nov;85(6):111145. doi: 10.1016/j.humimm.2024.111145. Epub 2024 Sep 20.
Transforming growth factor beta 1 (TGF-β1) is considered a pleotropic cytokine involved in the progression of cardiovascular disease. The purpose of the current study was to identify the relationship between serum levels of TGF-β1, the genetic variations (C509T rs1800469, T869C rs1800470) and their constructed haplotypes with the susceptibility of ischemic heart disease (IHD) in the Iraqi population.The case-control study enrolled 200 participants, including 100 patients with IHD and 100 healthy controls. Genotypes of (TGF-β1) polymorphisms were performed by TaqMan allele-specific probes detected by real-time PCR (RT-PCR). The serum level of TGF-β1 was measured by an ELISA assay. The obtained results showed that the two minor alleles of C509T rs1800469 and T869C rs1800470 were associated with a decreased risk of IHD, preventive fraction (PF%) = 11.6 %; 23.9 %, respectively. Genotype distribution was significantly noted in the TT genotype of C509T rs1800469 (p = 0.033) and in the TC genotype of T869C rs1800470 (p = 0.006) between patients and control groups. A significant distribution was seen in the C allele of T869C rs1800470 (p = 0.002) between the studied groups. The carriers of the TT genotype in the C509T rs1800469 and the TC; CC genotypes in the T869C rs1800470 decreased the chance of having IHD, (hazard ratio HR<1). Furthermore, the haplotype analysis observed that H1 (C-T) was significantly associated with the development of IHD (HR=1.66; 95 % CI=1.10-2.51; p = 0.021) and the inverse effect of H4 (T-C), (HR=0.36; 95 % CI=0.20-0.65; p = 0.001). The TGF-β1 alleles of both SNPs, TT genotype of C509T rs1800469, TC, CC of T869C rs1800470 and H4 locus of haplotypes were suggested to be protective biomarkers against IHD in Iraqi population.
转化生长因子β1（TGF-β1）被认为是一种参与心血管疾病进展的多效性细胞因子。本研究的目的是确定伊拉克人群中血清TGF-β1水平、基因变异（C509T rs1800469、T869C rs1800470）及其构建的单倍型与缺血性心脏病（IHD）易感性之间的关系。病例对照研究纳入了200名参与者，包括100例IHD患者和100名健康对照。通过实时荧光定量PCR（RT-PCR）检测的TaqMan等位基因特异性探针进行（TGF-β1）基因多态性的基因分型。通过酶联免疫吸附测定（ELISA）法测量TGF-β1的血清水平。获得的结果表明，C509T rs1800469和T869C rs1800470的两个次要等位基因与IHD风险降低相关，预防分数（PF%）分别为11.6%；23.9%。在患者组和对照组之间，C509T rs1800469的TT基因型（p = 0.033）和T869C rs1800470的TC基因型（p = 0.006）的基因型分布有显著差异。在研究组之间，T869C rs1800470的C等位基因分布有显著差异（p = 0.002）。C509T rs1800469的TT基因型携带者以及T869C rs1800470的TC；CC基因型携带者患IHD的几率降低，（风险比HR<1）。此外，单倍型分析观察到H1（C-T）与IHD的发生显著相关（HR = 1.66；95%可信区间CI = 1.10 - 2.51；p = 0.021），而H4（T-C）有相反的作用，（HR = 0.36；95%可信区间CI = 0.20 - 0.65；p = 0.001）。C509T rs1800469的T等位基因、T869C rs1800470的TC、CC基因型以及单倍型的H4位点的TGF-β1等位基因被认为是伊拉克人群中预防IHD的保护性生物标志物。
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