Lu Haiyan, Shao Junjun, Liu Wei, Gao Shandian, Zhou Guangqing, Ning Xiaoyu, Huang Haiyan, Liu Yijia, Chang Huiyun
State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, Gansu, 730046, PR China.
State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, Gansu, 730046, PR China.
Virus Res. 2024 Dec;350:199465. doi: 10.1016/j.virusres.2024.199465. Epub 2024 Sep 25.
This study aims to screen and identify linear B-cell epitopes on the structural proteins of African Swine Fever Virus (ASFV) to assist in the development of peptide-based vaccines. In experiments, 66 peptides of 12 structural proteins of ASFV were predicted as potential linear B-cell epitopes using bioinformatics tools and were designed; the potential epitope proteins carried the GST tag were expressed, purified, and subjected to antigenicity analysis with porcine antiserum against ASFV, and further identified based on their immunogenicity in mice. A total of 22 potential linear B-cell epitopes showed immunoreactivity and immunogenicity. Of these epitopes, 13 epitopes were firstly identified including 4 epitopes located in p72 (352-363, 416-434, 424-439, 496-530 aa), 3 epitopes located in pE248R (121-136, 138-169, 158-185 aa), and only one epitope of each protein of pH108R (33-46 aa), p17 (63-86 aa), pE120R (65-117 aa), pE199L (175-189 aa), p12 (36-56 aa) as well as pB438L (211-230 aa). Notably, the immunoreactivity of the epitopes from the 63-86 aa of p17 and the 65-117 aa of pE120R were the highest amongst identified epitopes, while the immunogenicity of epitopes from the 36-56 aa of p12, the 211-230 aa of pB438L, the 352-363 aa of p72 and the 63-86 aa of p17 were the best strong. The other 9 epitopes are partly overlapped with previous researches. These epitopes identified here will further enrich the database of ASFV epitope, as well as help to develop safe, effective epitope-based ASF vaccines and ASF diagnostic reagents.
本研究旨在筛选和鉴定非洲猪瘟病毒（ASFV）结构蛋白上的线性B细胞表位，以助力基于肽的疫苗开发。实验中，利用生物信息学工具预测并设计了ASFV 12种结构蛋白的66条肽段作为潜在线性B细胞表位；表达、纯化携带GST标签的潜在表位蛋白，并用猪抗ASFV抗血清进行抗原性分析，并基于其在小鼠中的免疫原性进一步鉴定。共有22个潜在线性B细胞表位表现出免疫反应性和免疫原性。在这些表位中，首次鉴定出13个表位，包括位于p72（352 - 363、416 - 434、424 - 439、496 - 530氨基酸）的4个表位、位于pE248R（121 - 136、138 - 169、158 - 185氨基酸）的3个表位，以及pH108R（33 - 46氨基酸）、p17（63 - 86氨基酸）、pE120R（65 - 117氨基酸）、pE199L（175 - 189氨基酸）、p12（36 - 56氨基酸）以及pB438L（211 - 230氨基酸）各蛋白的仅一个表位。值得注意的是，p17的63 - 86氨基酸和pE120R的65 - 117氨基酸处的表位免疫反应性在已鉴定表位中最高，而p12的36 - 56氨基酸、pB438L的211 - 230氨基酸、p72的352 - 363氨基酸和p17的63 - 86氨基酸处的表位免疫原性最强。其他9个表位与先前研究部分重叠。此处鉴定的这些表位将进一步丰富ASFV表位数据库，同时有助于开发安全、有效的基于表位的非洲猪瘟疫苗和非洲猪瘟诊断试剂。
