Binny Bincy, Joseph George, Venter Andre R
Department of Chemistry, Western Michigan University, Kalamazoo, Michigan 49008-5413, United States.
Anal Chem. 2024 Oct 1;96(39):15773-15779. doi: 10.1021/acs.analchem.4c03867. Epub 2024 Sep 22.
A previous comparative study between helium and nitrogen as nebulizing and desolvation gases in electrospray ionization (ESI) and desorption electrospray ionization (DESI) found that the signal responses of compounds of varying sizes and polarities were improved. Here, an expanded selection of nebulizing gases was evaluated to investigate mechanisms of improvement. The set of nebulizing gases included hydrogen, helium, nitrogen, argon, and carbon dioxide. Results indicate that the signal enhancements are achieved by gases lighter than nitrogen and that the previously described helium effects can be improved by using the more economical and sustainable hydrogen as a nebulizing gas. Additionally, H and He reduce the desorption footprint, which could be potentially useful in increasing the resolution of chemical imaging microscopy, especially since, despite the smaller footprint obtained using helium and hydrogen, higher signals are obtained compared to nitrogen.
先前一项关于在电喷雾电离（ESI）和解吸电喷雾电离（DESI）中使用氦气和氮气作为雾化和去溶剂化气体的比较研究发现，不同大小和极性的化合物的信号响应得到了改善。在此，对更多种类的雾化气体进行了评估，以研究改善的机制。雾化气体包括氢气、氦气、氮气、氩气和二氧化碳。结果表明，比氮气轻的气体可实现信号增强，并且通过使用更经济和可持续的氢气作为雾化气体，可以改善先前所述的氦气效应。此外，氢气和氦气减小了解吸足迹，这可能有助于提高化学成像显微镜的分辨率，特别是因为，尽管使用氦气和氢气获得的足迹较小，但与氮气相比，信号更高。
