Li Xin, Han Daobin, Li Xinmin, Zhou Chunjie, Shen Bo, Wei Honglu, Lou Qian, Liu Changjin, Chen Tingmei
Department of Laboratory Medicine, Chongqing Jiangjin District Hospital of Traditional Chinese Medicine, Chongqing 402284, China; Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
Department of Laboratory Medicine, Second Hospital of Shandong University, Shandong 250033, China.
Bioelectrochemistry. 2025 Feb;161:108819. doi: 10.1016/j.bioelechem.2024.108819. Epub 2024 Sep 18.
The construction of simple, stable, low-cost and reproducible enzyme-free electrochemical biosensors can effectively avoid the problem of signal attenuation caused by enzyme inactivation. Hererin, we prepared a novel nanoenzymes PdPtCu mesoporous nanocubes (MNCs) to construct a label-free sandwich electrochemical immunosensor for the highly sensitivity detection of HIV-p24. PdPtCu MNCs have excellent peroxidase activity against hydrogen peroxide (HO) due to their synergistic ternary composition, large surface area and ability to penetrate mesoporous channels. Moreover, highly conductive and biocompatible gold nanoparticles@graphene oxide (AuNPs@GO) was introduced as a substrate to modify a glassy carbon electrode (GCE). Owing to the excellent electrochemical performance of the PdPtCu MNCs and AuNPs@GO, the developed immunosensors exhibited a good linear response from 0.04 pg/mL to 100 ng/mL with a low detection limit of 20 fg/mL. In addition, the established method exhibited excellent practical performance in human serum. This novel strategy provides a promising platform for ultrasensitive detection of the HIV-p24 in the field of clinical diagnostics.
构建简单、稳定、低成本且可重复的无酶电化学生物传感器能够有效避免因酶失活导致的信号衰减问题。在此，我们制备了一种新型纳米酶PdPtCu介孔纳米立方体（MNCs），以构建用于高灵敏度检测HIV-p24的无标记夹心型电化学免疫传感器。由于其协同三元组成、大表面积以及穿透介孔通道的能力，PdPtCu MNCs对过氧化氢（HO）具有优异的过氧化物酶活性。此外，引入了高导电性和生物相容性的金纳米颗粒@氧化石墨烯（AuNPs@GO）作为基底来修饰玻碳电极（GCE）。由于PdPtCu MNCs和AuNPs@GO具有优异的电化学性能，所开发的免疫传感器在0.04 pg/mL至100 ng/mL范围内呈现出良好的线性响应，检测限低至20 fg/mL。此外，所建立的方法在人血清中表现出优异的实际性能。这种新颖的策略为临床诊断领域中HIV-p24的超灵敏检测提供了一个有前景的平台。
