Li Rui, Que Wenzhong, Han Haiyan, Shi Honghui, Wang Rongfeng, Qiu Bin
Department of Dermatology, Fuzhou First General Hospital, Fuzhou, China.
Department of Rheumatology, Fuzhou First General Hospital, Fuzhou, China.
Mikrochim Acta. 2025 Apr 17;192(5):308. doi: 10.1007/s00604-025-07064-6.
IL-17 (interleukin-17) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases; it is thus very necessary to design an effective method for monitoring IL-17. Herein, by preparing silver nanoparticles/graphdiyne (Ag/GDY) with simple electroless deposition as the sensing platform and methylene blue/UiO-66-NH MOFs (MB/MOFs) as the signaling-amplifier, a highly efficient sandwich-like electrochemical immunosensing (SLEI) platform was designed for IL-17 detection. The superior electrical property, easy preparation, large surface area, and high stability of Ag/GDY make it a desirable substrate to immobilize primary antibody of IL-17. Meanwhile, the unique structure of UiO-66-NH is beneficial to immobilize MB probe and secondary antibody. By optimizing experimental conditions, the MB current obtained at the as-designed SLEI platform increases along with IL-17 increase, and the related linearity is 1.0 pg mL-1.0 ng mL coupled with a low detection limit of 0.3 pg mL. It is expected that the SLEI platform based on Ag/GDY and MB/MOFs shows some potential application for IL-17 detection.
白细胞介素-17（IL-17）是一种常见的促炎细胞因子，可作为多种疾病的重要生物标志物；因此，设计一种监测IL-17的有效方法非常必要。在此，通过以简单的化学沉积法制备银纳米颗粒/石墨炔（Ag/GDY）作为传感平台，并以亚甲基蓝/UiO-66-NH金属有机框架（MB/MOFs）作为信号放大器，设计了一种用于检测IL-17的高效夹心式电化学免疫传感（SLEI）平台。Ag/GDY优异的电学性能、易于制备、大表面积和高稳定性使其成为固定IL-17一抗的理想基底。同时，UiO-66-NH独特的结构有利于固定MB探针和二抗。通过优化实验条件，在设计的SLEI平台上获得的MB电流随IL-17的增加而增加，相关线性范围为1.0 pg mL-1.0 ng mL，检测限低至0.3 pg mL。基于Ag/GDY和MB/MOFs的SLEI平台有望在IL-17检测中展现出一定的潜在应用价值。
