小鼠对星形诺卡菌蛋白质、碳水化合物和脂质组分的免疫反应。

Immunological responses to protein, carbohydrate and lipid fractions of Nocardia asteroides in mice.

作者信息

Gupta R, Pancholi V, Vinayak V K, Khuller G K

出版信息

J Med Microbiol. 1985 Oct;20(2):263-74. doi: 10.1099/00222615-20-2-263.

Abstract

The protein, polysaccharide and phospholipid constituents of Nocardia asteroides have been partially purified and their immunogenicity studied in mice. Humoral and cellular immune responses were demonstrated against a crude cytoplasmic protein fraction (CPF). Two fractions of CPF were prepared on a Sephadex G-200 column; a high mol. wt fraction, fraction-1(F1) was capable of eliciting both types of immune responses, whereas fraction-2(F2) behaved more like a hapten. Phosphatides elicited only humoral responses whereas polysaccharides were non-immunogenic.

摘要

星状诺卡菌的蛋白质、多糖和磷脂成分已被部分纯化，并在小鼠中研究了它们的免疫原性。针对粗制细胞质蛋白组分（CPF）证实了体液免疫和细胞免疫反应。在Sephadex G-200柱上制备了CPF的两个组分；高分子量组分，即组分1（F1）能够引发两种类型的免疫反应，而组分2（F2）的行为更像半抗原。磷脂仅引发体液反应，而多糖无免疫原性。