State Key Laboratory of Tea Plant Biology and Utilization, Anhui Provincial Key Laboratory of Food Safety Monitoring and Quality Control, School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, PR China; Joint Research Center for Food Nutrition and Health of IHM, Anhui Agricultural University, Hefei 230036, Anhui, PR China.
The First Aliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, China.
Int J Biol Macromol. 2024 Nov;280(Pt 2):135846. doi: 10.1016/j.ijbiomac.2024.135846. Epub 2024 Sep 21.
Yeast β-glucan intervention offers a promising strategy for managing colitis; however, the mechanisms remain unknown. In the present work, the protective effects of yeast β-glucan on DSS-induced colitis in mice was evaluated, focusing on its interaction with gut microbiota. The result showed yeast β-glucan significantly alleviated colitis symptoms, evidenced by reduced weight loss, lower disease activity index (DAI) scores, and minimized intestinal damage. It enhanced intestinal barrier integrity via upregulation of tight junction proteins, suppressed lipopolysaccharide (LPS) release, and decreased pro-inflammatory cytokines production. Additionally, yeast β-glucan boosted short-chain fatty acids (SCFAs) production, and activated their receptors, increased the relative abundances of beneficial microbes like Lactobacillus and Lachnospiraceae_UCG-006. Transcriptomic analyses suggest that yeast β-glucan mitigates inflammation by downregulating gene expression related to IL-17 pathway. Our findings highlight potential of yeast β-glucan as a therapeutic agent for colitis through modulation of gut microbiota and inflammatory responses.
酵母β-葡聚糖干预为治疗结肠炎提供了一种有前途的策略；然而，其机制尚不清楚。本研究评价了酵母β-葡聚糖对 DSS 诱导的结肠炎小鼠的保护作用，重点关注其与肠道微生物群的相互作用。结果表明，酵母β-葡聚糖显著缓解了结肠炎症状，表现在体重减轻减少、疾病活动指数（DAI）评分降低和肠道损伤最小化。它通过上调紧密连接蛋白来增强肠道屏障完整性，抑制脂多糖（LPS）释放，并减少促炎细胞因子的产生。此外，酵母β-葡聚糖促进短链脂肪酸（SCFAs）的产生，并激活其受体，增加有益微生物如乳杆菌和lachnospiraceae_UCG-006 的相对丰度。转录组分析表明，酵母β-葡聚糖通过下调与 IL-17 途径相关的基因表达来减轻炎症。我们的研究结果强调了酵母β-葡聚糖作为治疗结肠炎的一种治疗剂的潜力，通过调节肠道微生物群和炎症反应。
