Yu Shangjie, Zhang Jiani, Cao Yinxing, Zhong Chunmei, Xie Jun
Institute of Biomass Engineering, South China Agricultural University, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Guangzhou 510642, PR China.
Institute of Biomass Engineering, South China Agricultural University, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Guangzhou 510642, PR China.
Int J Biol Macromol. 2024 Sep 21;280(Pt 3):135788. doi: 10.1016/j.ijbiomac.2024.135788.
Dioscorea composita (D. composita) is a perennial herb with abundant steroidal saponins that have gained worldwide attention for their remarkable efficacy in cardiovascular diseases. However, few studies have been worked on the regulatory network of steroidal saponins biosynthesis under phytohormone induced. In this study, we combined the transcriptome and metabolome analysis to reveal the variation of diosgenin and steroidal saponins in transcriptional and metabolism levels under methyl-jasmonate (MeJA) treatment. Although the application of MeJA indeed significantly increased the accumulation of diosgenin of D. composita, different types of steroidal saponins exhibited different accumulation patterns. Consistently, the expression levels of UDP-glycosyltransferases and Cytochrome P450 monooxygenases genes that highly related to the accumulation of steroidal saponins were either up- or down-regulated. Correlation analyses of transcription factors (TFs)-steroidal saponins and structural genes-TFs were further to identified the TFs potentially involved in the regulation of steroidal saponins biosynthesis. Silencing of DcWRKY11 in Dioscorea composita decreases the accumulation of steroidal saponins by regulating the expression steroidal saponins synthesis genes, suggesting that DcWRKY11 is a positive regulator in the regulation of steroidal saponins biosynthesis. Our findings take a deeper understanding of the regulatory network of MeJA-mediated steroidal saponins biosynthesis in D. composita.
盾叶薯蓣（Dioscorea composita）是一种多年生草本植物，含有丰富的甾体皂苷，其在心血管疾病方面的显著功效已引起全球关注。然而，关于植物激素诱导下甾体皂苷生物合成调控网络的研究较少。在本研究中，我们结合转录组和代谢组分析，以揭示茉莉酸甲酯（MeJA）处理下薯蓣皂苷元和甾体皂苷在转录水平和代谢水平上的变化。虽然MeJA的施用确实显著增加了盾叶薯蓣中薯蓣皂苷元的积累，但不同类型的甾体皂苷表现出不同的积累模式。同样，与甾体皂苷积累高度相关的尿苷二磷酸糖基转移酶和细胞色素P450单加氧酶基因的表达水平上调或下调。进一步对转录因子（TFs）-甾体皂苷和结构基因-TFs进行相关性分析，以鉴定可能参与甾体皂苷生物合成调控的TFs。在盾叶薯蓣中沉默DcWRKY11会通过调节甾体皂苷合成基因的表达来降低甾体皂苷的积累，这表明DcWRKY11是甾体皂苷生物合成调控中的一个正向调节因子。我们的研究结果有助于更深入地了解MeJA介导的盾叶薯蓣甾体皂苷生物合成调控网络。
