Zhang Jie-Qi, Fu Xiao-Mei, Zhuo Yue, Yang Jin-Na, Hao Ning, Wang Wen-Jia, Hu Yun-Hui, Fang Jian-Song
Science and Technology Innovation Center of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405, China.
Tasly Pharmaceutical Group Co., Ltd. Tianjin 300410, China National Key Laboratory of Chinese Medicine Modernization Tianjin 300193, China.
Zhongguo Zhong Yao Za Zhi. 2024 Sep;49(17):4672-4686. doi: 10.19540/j.cnki.cjcmm.20240426.301.
The main chemical components of Yangxue Qingnao Wan(YXQNW) were analyzed and identified by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS). According to the mass spectrometry information, Mass Hunter 10.0 analysis software was used to compare the collected quasi-molecular ion peaks and secondary fragment ions with literature and reference substances. A total of 131 compounds were identified from YXQNW, including 11 phenylpropanoids, 11 flavonoids, 42 nitrogen-containing compounds, 12 terpenoids, 17 phthalides, 23 quinones, and 15 other compounds. The anti-aging activity of YXQNW and six compounds from YXQNW, including rosmarinic acid, gallic acid, rutin, umbelliferone, hyperoside, and vanillic acid, were evaluated by D-galactose(D-gal)-induced HT22 cell senescence model. The effects of the compounds on HT22 cell damage and individual cell proliferation ability were observed from overall and individual perspectives by the Beyo Click~(TM) EdU-555 cell proliferation kit, and apoptosis was detected by the Annexin V-FITC/PI double staining apoptosis detection kit. Finally, the anti-aging effect of the compounds was tested by a cell senescence β-galactosidase staining kit. This study provides a more comprehensive analysis of the chemical components of YXQNW and evaluates its anti-aging effect, which will provide a scientific basis for basic research on the efficacy of YXQNW for the treatment of various neurological diseases, such as Alzheimer's disease(AD), headache, and memory loss.
采用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF-MS/MS）对养血清脑丸（YXQNW）的主要化学成分进行分析和鉴定。根据质谱信息，使用Mass Hunter 10.0分析软件将采集到的准分子离子峰和二级碎片离子与文献及对照品进行比较。从养血清脑丸中总共鉴定出131种化合物，包括11种苯丙素类、11种黄酮类、42种含氮化合物、12种萜类、17种酞类、23种醌类和15种其他化合物。通过D-半乳糖（D-gal）诱导的HT22细胞衰老模型，评价了养血清脑丸及其6种成分（迷迭香酸、没食子酸、芦丁、伞形花内酯、金丝桃苷和香草酸）的抗衰老活性。使用Beyo Click™ EdU-555细胞增殖试剂盒从整体和个体角度观察这些化合物对HT22细胞损伤和个体细胞增殖能力的影响，并使用Annexin V-FITC/PI双染凋亡检测试剂盒检测细胞凋亡。最后，使用细胞衰老β-半乳糖苷酶染色试剂盒检测这些化合物的抗衰老作用。本研究对养血清脑丸的化学成分进行了更全面的分析，并评价了其抗衰老作用，为养血清脑丸治疗阿尔茨海默病（AD）、头痛和记忆力减退等各种神经系统疾病的疗效基础研究提供了科学依据。
