Jiang Cheng, Zhao Chunyan, Gao Hesheng, Gu Ziqi, Wu Yuehong, Wen Shuangshuang, Chen Ningning, An Yi, Huang Lichao, Lu Yongquan, Zhang Jin, Lu Meng-Zhu
State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China.
Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou, China.
Plant Cell Environ. 2025 Jan;48(1):634-648. doi: 10.1111/pce.15156. Epub 2024 Sep 22.
Plant organs achieve their specific size and shape through the coordination of cell division and cell expansion, processes that are profoundly influenced by environmental cues. Cytokinesis during cell division depends on the position of the cytokinetic wall, but how this process responses to environment fluctuations remains underexplored. Here, we investigated a regulatory module involving C2H2-type zinc finger protein (C2H2-ZFP) in leaf morphology during drought stress. A total of 123 C2H2-ZFP members were identified through a comparative genome survey in Populus alba × P. glandulosa '84K'. Among them, PagSUPa, an orthologous gene of Arabidopsis SUPERMAN, was selected due to its responsiveness to drought stress and was further confirmed to play a role in leaf development. Phenotypic characterization and cellular analysis revealed that PagSUPa fine-tunes the duration of cell proliferation in the adaxial epidermis, thereby influencing leaf morphology by modulating leaf adaxial-abaxial polarity. Additionally, we found that PagSUPa directly suppresses the expression of PHRAGMOPLAST ORIENTING KINESIN1 (PagPOK1) and PagPOK2, genes encoding proteins involved in phragmoplast orientation and position, which results in impaired cytokinesis and cell wall organization. This study provides novel insights into the regulatory network governed by the SUP gene during leaf development, specifically in relation to cell division.
植物器官通过细胞分裂和细胞扩张的协调来实现其特定的大小和形状，而这些过程会受到环境信号的深刻影响。细胞分裂过程中的胞质分裂取决于胞质分裂壁的位置，但该过程如何响应环境波动仍未得到充分研究。在这里，我们研究了干旱胁迫期间涉及C2H2型锌指蛋白（C2H2-ZFP）的一个调控模块在叶片形态中的作用。通过对银白杨×腺毛杨‘84K’进行比较基因组调查，共鉴定出123个C2H2-ZFP成员。其中，拟南芥SUPERMAN的直系同源基因PagSUPa，因其对干旱胁迫的响应而被选中，并进一步证实其在叶片发育中发挥作用。表型特征和细胞分析表明，PagSUPa可微调近轴表皮细胞增殖的持续时间，从而通过调节叶片近轴-远轴极性来影响叶片形态。此外，我们发现PagSUPa直接抑制PHRAGMOPLAST ORIENTING KINESIN1（PagPOK1）和PagPOK2的表达，这两个基因编码参与成膜体定向和定位的蛋白质，从而导致胞质分裂和细胞壁组织受损。这项研究为SUP基因在叶片发育过程中，特别是与细胞分裂相关的调控网络提供了新的见解。
