转录因子 PagERF110 通过直接调控杨树中的 PagHB16 抑制叶片发育。

Transcription factor PagERF110 inhibits leaf development by direct regulating PagHB16 in poplar.

机构信息

College of Horticulture and Landscape Architecture, Yangtze University, Jingzhou, China.

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.

出版信息

Plant Sci. 2025 Jan;350:112309. doi: 10.1016/j.plantsci.2024.112309. Epub 2024 Oct 28.

DOI:10.1016/j.plantsci.2024.112309

PMID:39490445

Abstract

Ethylene-responsive factor (ERF) family genes are crucial for plant growth and development. This study analyzed the functional role of the PagERF110 gene in leaf development of Populus alba×P. glandulosa. PagERF110 contains the AP2 conserved domain and exhibits transcriptional activation activity at its C-terminus. Overexpression of PagERF110 in transgenic poplar trees resulted in reduced leaf size, leaf area, and vein xylem thickness. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) experiments confirmed that PagERF110 interacts with PagACD32.1. Transcriptome sequencing revealed that PagERF110 regulates the expression of key genes involved in leaf development. Furthermore, yeast one-hybrid (Y1H) assays, GUS staining, and ChIP experiments collectively confirmed that PagERF110 targets the expression of PagHB16. In summation, our findings demonstrate that PagERF110 functions as a negative regulator in poplar leaf development.

摘要

乙烯响应因子（ERF）家族基因对于植物的生长和发育至关重要。本研究分析了 PagERF110 基因在银腺杨×青杨杂种叶片发育中的功能作用。PagERF110 包含 AP2 保守结构域，其 C 端具有转录激活活性。在转基因杨树上过表达 PagERF110 导致叶片变小、叶面积减小和叶脉木质部厚度变薄。酵母双杂交（Y2H）和双分子荧光互补（BiFC）实验证实 PagERF110 与 PagACD32.1 相互作用。转录组测序表明 PagERF110 调控与叶片发育相关的关键基因的表达。此外，酵母单杂交（Y1H）测定、GUS 染色和 ChIP 实验共同证实 PagERF110 靶向 PagHB16 的表达。总之，我们的研究结果表明 PagERF110 作为一个负调控因子在杨树叶片发育中发挥作用。