Faculty of Veterinary Medicine, Omar Al-Mukhtar University, Al-Bayda, Libya.
Agricultural Research Center, Al-Bayda, Libya.
Open Vet J. 2024 Aug;14(8):1843-1849. doi: 10.5455/OVJ.2024.v14.i8.12. Epub 2024 Aug 31.
The most common natural prion disease that primarily affects sheep and goats is scrapie. It belongs to a group of disorders known as transmissible spongiform encephalopathies, which impact both humans and animals.
The research is aimed to examine and confirm the presence of scrapie in Libya using immunohistochemistry (IHC) techniques.
Brain samples were collected from thirty-three sheep older than two years of age showing clinical signs resembling to scrapie during the period between 2018 and 2023, regardless of race or gender. Three animals, six months old, healthy, and without any symptoms, were used as negative controls. Different parts of the brain, including the obex and cerebellum, were taken from each case. The IHC technique used in this study involved staining with monoclonal antibody L42 and DAB (3,3'-diaminobenzidine) as a chromogenic substrate.
The IHC examination showed the expression of prion proteins in brain tissue in twenty-three samples. The staining intensity was markedly observed in the neuronal cell bodies and around blood vessels.
The findings of this study provide evidence that scrapie exists in Libya.
最常见的主要影响绵羊和山羊的天然朊病毒病是羊瘙痒病。它属于一组被称为传染性海绵状脑病的疾病，既影响人类也影响动物。
本研究旨在使用免疫组织化学（IHC）技术检查并确认利比亚存在羊瘙痒病。
在 2018 年至 2023 年期间，采集了 33 只年龄大于两岁且出现类似于羊瘙痒病临床症状的绵羊的脑组织样本，无论其种族或性别如何。此外，还使用了 3 只 6 个月大、健康且无任何症状的动物作为阴性对照。从每个病例中取出脑的不同部位，包括延髓和小脑。本研究中使用的 IHC 技术涉及用单克隆抗体 L42 和 DAB（3,3'-二氨基联苯胺）作为显色底物进行染色。
免疫组织化学检查显示 23 个样本的脑组织中存在朊病毒蛋白的表达。在神经元细胞体和血管周围观察到明显的染色强度。
本研究的结果提供了利比亚存在羊瘙痒病的证据。
