Huang Pengyu, He Yongan, Huang Chunnuan, Jiang Shuhan, Gan Ji, Wu Rong, Ai Chengjiao, Huang Jiean, Yao Chaoguang, Chen Quanzhi
School of Basic Medical Sciences, The Second Affiliated Hospital, Guangxi Medical University Nanning 530021 Guangxi P. R. China
Department of Gastroenterology, People's Hospital of Guangxi Zhuang Autonomous Region Nanning 530021 Guangxi P. R. China.
RSC Adv. 2024 Sep 20;14(41):30055-30069. doi: 10.1039/d4ra04546g. eCollection 2024 Sep 18.
In this study, a metal-organic framework (MOF) antimicrobial gel loaded with platelet-rich plasma (PRP) was prepared to improve the biological properties of gelatin gels and enhance their wound healing efficiency. PRP, MOF particles, and PRP-loaded MOF particles were each integrated into gelatin gels. The performance of the gels was evaluated for micro-structure, mechanical strength, bio-compatibility and pro-wound healing effects. The results revealed that the integration of PRP created a multi-cross-linked structure, increasing the ductility of the gels by over 40%. The addition of MOF particles significantly increased the strength of the gel from 13 kPa to 43 kPa. The combination of MOF and PRP further improved the cell induction and migration capabilities of the composite gel, and the scratches in the PRP/MOF@GelMA group had completely healed within 48 h. Due to the presence of MOF and PRP, the gel dressing exhibited inhibitory effects of 45.7% against () and 50.2% against (). Different gels promoted tissue regeneration and wound healing ability of bacterial-infected wounds in C57 rats, while PRP/MOF@GelMA showed the strongest wound repair ability with 100% healing. This study provides a new strategy for the development and clinical application of gel dressings.
在本研究中，制备了一种负载富血小板血浆（PRP）的金属有机框架（MOF）抗菌凝胶，以改善明胶凝胶的生物学性能并提高其伤口愈合效率。将PRP、MOF颗粒和负载PRP的MOF颗粒分别整合到明胶凝胶中。对凝胶的微观结构、机械强度、生物相容性和促伤口愈合效果进行了评估。结果表明，PRP的整合形成了多交联结构，使凝胶的延展性提高了40%以上。MOF颗粒的添加显著提高了凝胶的强度，从13 kPa提高到43 kPa。MOF和PRP的组合进一步提高了复合凝胶的细胞诱导和迁移能力，PRP/MOF@GelMA组的划痕在48小时内完全愈合。由于MOF和PRP的存在，凝胶敷料对（）的抑制率为45.7%，对（）的抑制率为50.2%。不同的凝胶促进了C57大鼠细菌感染伤口的组织再生和伤口愈合能力，而PRP/MOF@GelMA表现出最强的伤口修复能力，愈合率为100%。本研究为凝胶敷料的开发和临床应用提供了一种新策略。
