Batsford S R, Weghaupt R, Takamiya H, Vogt A
Nephron. 1985;41(2):146-51. doi: 10.1159/000183570.
The influence of certain physicochemical and biologic properties of protein antigens on their handling by the glomerular mesangium was studied in rats. The following ferritin-based probes were employed: (a) naturally occurring ferritin isomers to examine the role of molecular size; (b) chemically cationized ferritins (pI 7.1 and 8.8) to test charge effects; (c) glutaraldehyde (GA-)coupled ferritin as an analogue of a toxic molecule. Molecular size was found to be a major determinant of the extent and rapidity of antigen uptake, but had only a minor influence on persistence. The rapidity of uptake and antigen persistence was charge dependent, but to a limited extent. Biologic activity of macromolecules appears to be a very important determinant of mesangial handling since GA-treated ferritin was taken up much more rapidly and to a greater extent than native ferritin, and could persist for very prolonged periods.
在大鼠中研究了蛋白质抗原的某些物理化学和生物学特性对肾小球系膜处理这些抗原的影响。使用了以下基于铁蛋白的探针：（a）天然存在的铁蛋白异构体，以研究分子大小的作用；（b）化学阳离子化铁蛋白（pI 7.1和8.8），以测试电荷效应；（c）戊二醛（GA-）偶联铁蛋白，作为有毒分子的类似物。发现分子大小是抗原摄取程度和速度的主要决定因素，但对其持久性影响较小。摄取速度和抗原持久性取决于电荷，但程度有限。大分子的生物学活性似乎是系膜处理的一个非常重要的决定因素，因为经GA处理的铁蛋白比天然铁蛋白摄取更快、程度更大，并且可以持续很长时间。
