Analyzing Emulsion Dynamics via Direct Visualization and Statistical Methodologies.

Analytical centrifugation is a powerful technique that leverages the principles of centrifugal force and optical detection to characterize emulsion droplets in a label-free and high-throughput manner. Other advantages include minimal sample preparation effort and compatibility with a wide range of emulsion formulations. However, the resulting data can be rather complex and, thus, difficult to fully understand and interpret. To tackle this, we developed two analytical methodologies that enable an easy and intuitive understanding of the data as well as an objective, quantitative analysis and validated them using six model emulsions employing different surfactants. Through their application, insights with unprecedented clarity into dynamic emulsion behavior, stability mechanisms, and emulsion-based processes can be gained, facilitating advancements in fields such as food science, pharmaceuticals, and materials engineering.