Lavanya S, Ahmed Shafath, Nandini Vidyashree V, Saravanan Abinaya, Kr Sadhana, G Manjula
Prosthodontics, SRM Kattankulathur Dental College and Hospital (SRMIST), Chengalpattu, IND.
Cureus. 2024 Aug 20;16(8):e67348. doi: 10.7759/cureus.67348. eCollection 2024 Aug.
Objective The objective of this in vitro study was to comparatively evaluate the antifungal and physical properties of tissue conditioner incorporated with nanoparticles (NPs) of different types and concentrations. Materials and methods A total of 198 tissue conditioner samples were used in this study. The samples were categorized into a control group, namely, tissue conditioner without NPs (Group 1), and test groups, namely, tissue conditioner incorporated with zinc oxide (ZnO) NPs (Group 2) and magnesium oxide (MgO) NPs (Group 3). The antifungal properties and surface roughness of the samples were evaluated. The groups were further subdivided into seven subgroups: control (without NPs), 5% ZnO NPs, 10% ZnO NPs, 15% ZnO NPs, 3% MgO NPs, 5% MgO NPs, and 7% MgO NPs by weight. Surface roughness was measured using an optical profilometer, and antifungal activity was measured in terms of the diameter of the inhibition zone (DIZ) using the well diffusion method over seven days. Results The result showed that the 5% ZnO NPs subgroup had the lowest mean surface roughness, whereas the 15% ZnO NPs subgroup had the highest antifungal activity. Increasing the concentration of NPs increased the antifungal property, and there was a steady decrease in DIZ from day one to day seven in all test groups. Conclusion Our results showed that the incorporation of various concentrations of ZnO and MgO NPs into tissue conditioner samples positively affected their physical and antifungal properties. The highest antifungal activity was found in the 15% ZnO NPs subgroup, and the lowest surface roughness was found in the 5% ZnO NPs subgroup.
目的 本体外研究的目的是比较评估掺入不同类型和浓度纳米颗粒（NPs）的组织调理剂的抗真菌性能和物理性能。材料与方法 本研究共使用了198个组织调理剂样品。样品分为一个对照组，即不含NPs的组织调理剂（第1组），以及试验组，即掺入氧化锌（ZnO） NPs的组织调理剂（第2组）和氧化镁（MgO） NPs的组织调理剂（第3组）。评估了样品的抗真菌性能和表面粗糙度。这些组进一步细分为七个亚组：按重量计分别为对照组（不含NPs）、5% ZnO NPs、10% ZnO NPs、15% ZnO NPs、3% MgO NPs、5% MgO NPs和7% MgO NPs。使用光学轮廓仪测量表面粗糙度，并采用平板扩散法在七天内通过抑菌圈直径（DIZ）测量抗真菌活性。结果 结果表明，5% ZnO NPs亚组的平均表面粗糙度最低，而15% ZnO NPs亚组的抗真菌活性最高。增加NPs的浓度可提高抗真菌性能，并且在所有试验组中，从第一天到第七天DIZ稳步下降。结论 我们的结果表明，将不同浓度的ZnO和MgO NPs掺入组织调理剂样品中对其物理性能和抗真菌性能产生了积极影响。在15% ZnO NPs亚组中发现了最高的抗真菌活性，在5% ZnO NPs亚组中发现了最低的表面粗糙度。
