霍乱毒素对营养不良大鼠肠道腺苷酸环化酶的刺激作用。

Stimulation of intestinal adenylate cyclase by cholera toxin in malnourished rats.

作者信息

Pérez-Schael I, Flores J

出版信息

Proc Soc Exp Biol Med. 1985 Nov;180(2):231-5. doi: 10.3181/00379727-180-42169.

Abstract

The stimulation of intestinal adenylate cyclase by cholera toxin (CT) was studied in normal and malnourished rats 4 to 24 hr after a 30-min incubation of intestinal loops with the toxin. Whereas in control rats the enzyme activity returned to basal levels after 12 hr of incubation, in malnourished rats the activity of the enzyme remained significantly elevated even after 24 hr of the initial incubation. Malnourished animals had a reduced turnover rate of intestinal cells as determined by thymidine kinase activity. The delayed turnover of intoxicated cells may account for continuous activation of mucosal adenylate cyclase and possibly for prolongation of diarrhea in malnutrition.

摘要

在正常和营养不良的大鼠中，研究了霍乱毒素（CT）对肠腺苷酸环化酶的刺激作用。将肠袢与毒素孵育30分钟后，在4至24小时内进行观察。在对照大鼠中，孵育12小时后酶活性恢复到基础水平，而在营养不良的大鼠中，即使在最初孵育24小时后，酶活性仍显著升高。通过胸苷激酶活性测定，营养不良的动物肠细胞更新率降低。中毒细胞更新延迟可能是黏膜腺苷酸环化酶持续激活的原因，也可能是营养不良时腹泻持续时间延长的原因。