Lönnroth I, Lange S
Acta Pathol Microbiol Immunol Scand B. 1983 Dec;91(6):395-400. doi: 10.1111/j.1699-0463.1983.tb00066.x.
Clostridium difficile produces one diarrhoeogenic toxin designed A, and one cytopathogenic toxin designed B. Toxin A was purified in a four-step-fractionation procedure. In the last purification step the toxin was separated by elution with galactose from an agarose gel. The purified toxin A induced a clear and watery hypersecretion in intestinal loops of mouse, while mixtures of toxin A and B induced a haemorrhagic secretion. At an ED50 value for the purified toxin A of 0.5 microgram there was a brief, optimal hypersecretion after four hours. Like the fluid secretion induced by cholera toxin, that induced by toxin A could be inhibited by chlorpromazine or by depletion of intestinal bile. In contrast to cholera toxin, however, toxin A did not activate intestinal adenylate cyclase--at least not permanently. Antisera which neutralized cholera toxin did not neutralize toxin A, and vice versa.
艰难梭菌产生一种被称为A的致腹泻毒素和一种被称为B的细胞致病毒素。毒素A通过四步分级分离程序进行纯化。在最后一步纯化中，毒素通过用半乳糖从琼脂糖凝胶上洗脱而分离出来。纯化的毒素A在小鼠肠袢中诱导出清晰且水样的高分泌，而毒素A和B的混合物则诱导出出血性分泌。纯化毒素A的半数有效剂量（ED50）为0.5微克时，四小时后会出现短暂的最佳高分泌。与霍乱毒素诱导的液体分泌一样，毒素A诱导的分泌可被氯丙嗪或肠道胆汁耗尽所抑制。然而，与霍乱毒素不同的是，毒素A不会激活肠道腺苷酸环化酶——至少不会永久激活。中和霍乱毒素的抗血清不会中和毒素A，反之亦然。
