Beyer-Mears A, Kelly K, Cruz E
Pharmacology. 1985;31(3):170-9. doi: 10.1159/000138111.
Sorbinil, an aldose reductase inhibitor, in combination with diet normalization, arrested stage-II galactose cataract and restored lens transparency. During the reversal process, determination of lens dry weight, dulcitol and myo-inositol content as well as individual fiber cell ultrastructure offered a comprehensive index of lens integrity. In this study, young rats received a 50% galactose diet for 10 days to produce a stage-II sugar cataract. Then they were placed on one of the following diets: 50% galactose and Sorbinil (20 mg/kg); 50% galactose; normal diet, normal diet and Sorbinil (20 mg/kg). From each group, equal numbers were sacrificed at 5, 10 and 20 days. Although differences were obtained after 5 and 10 days, the 20-day reversal period provided the most significant findings. Only the combination of Sorbinil and normal diet restored lens transparency, normalized lens myo-inositol content and dry weight and partially restored fiber cell integrity as evidenced by diminished granulation and increased fiber synthesis. Neither Sorbinil treatment during galactose administration nor normal diet alone were sufficient to protect against further cataractogenesis, thus indicating a synergistic effect of Sorbinil in combination with normal diet.
醛糖还原酶抑制剂索比尼尔与饮食调整相结合，可阻止二期半乳糖性白内障的发展，并恢复晶状体透明度。在逆转过程中，测定晶状体干重、山梨醇和肌醇含量以及单个纤维细胞的超微结构，可提供晶状体完整性的综合指标。在本研究中，幼鼠接受50%半乳糖饮食10天以形成二期糖性白内障。然后将它们置于以下饮食之一：50%半乳糖和索比尼尔（20毫克/千克）；50%半乳糖；正常饮食；正常饮食和索比尼尔（20毫克/千克）。每组在第5、10和20天处死等量动物。虽然在第5天和第10天后出现了差异，但20天的逆转期提供了最显著的结果。只有索比尼尔与正常饮食的组合恢复了晶状体透明度，使晶状体肌醇含量和干重正常化，并部分恢复了纤维细胞完整性，表现为颗粒化减少和纤维合成增加。在给予半乳糖期间单独使用索比尼尔治疗或单独使用正常饮食均不足以防止进一步的白内障形成，因此表明索比尼尔与正常饮食联合具有协同作用。
