使用大鼠和土拨鼠S9制剂在沙门氏菌/微粒体试验中的比较致突变性测试。
Comparative mutagenicity tests in the Salmonella/microsome assay with rat and woodchuck S9 preparations.
作者信息
Rashid K A, Babish J G, Johnson B E, Mumma R O
出版信息
Toxicology. 1985 Aug;36(2-3):139-46. doi: 10.1016/0300-483x(85)90048-4.
The Salmonella mutagenicity assay was utilized to compare the hepatic S9 fractions from untreated and 3-methylcholanthrene (MC) induced woodchucks with Aroclor 1254 induced rats. Three known promutagens, benzo[a]pyrene (BP), 7,12-dimethylbenz[a]anthracene (DMBA), and 2-aminofluorene (AF) were tested at 5 concentrations with the strain TA100 against 3 levels of S9 fraction. Both woodchuck S9 fractions were as effective as the rat S9 in activating BP and both were more effective than the rat S9 in activating DMBA. Untreated woodchuck S9 was also as effective as rat S9 in activating AF. The protein content of the S9 fraction did not differ significantly between rats and woodchucks, but the P-450 content of the rat S9 was approximately 3.5 times that of woodchuck.
利用沙门氏菌致突变性试验，比较未处理的和经3-甲基胆蒽（MC）诱导的土拨鼠与经多氯联苯混合物1254诱导的大鼠的肝脏S9组分。用菌株TA100在5种浓度下针对3种水平的S9组分测试了三种已知的前诱变剂，即苯并[a]芘（BP）、7,12-二甲基苯并[a]蒽（DMBA）和2-氨基芴（AF）。两种土拨鼠S9组分在激活BP方面与大鼠S9一样有效，且在激活DMBA方面都比大鼠S9更有效。未处理的土拨鼠S9在激活AF方面也与大鼠S9一样有效。大鼠和土拨鼠的S9组分蛋白质含量没有显著差异，但大鼠S9的P-450含量约为土拨鼠的3.5倍。
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