Csonka E, Somogyi A, Augustin J, Haberbosch W, Schettler G, Jellinek H
Virchows Arch A Pathol Anat Histopathol. 1985;407(4):441-7. doi: 10.1007/BF00709990.
Monolayers of endothelial, smooth muscle and fibroblastic cells of healthy porcine, bovine or human fetal origin were treated with 10(-4) to 10(-9) M final concentrations of nicotine. The effect was registered as changes in the synthesis and polyimerization of the cytoskeleton. The silver and gold impregnation method produced anisotropy of the synthetic granules and of the final polymers of microtubules and filaments under physiological conditions as revealed by polarization microscopy. Since the orientation of the cells was inhomogeneous in the cultures, the organization of the orientation was expressed as the sum of alternative diagonal and orthogonal measuring of anisotropy by a computerized microraster morphometry system joined to an OPTON cytophotometer. The 8-day-old control and treated cultures were also examined by electronmicroscopy. Nicotine stimulated the synthesis and polyimerization of the cytoskeletal protein. This phenomenon is evident in smooth muscle cells, and partly also in endothelium. Fibroblasts were not influenced by the doses of nicotine tested.
将来自健康猪、牛或人类胎儿的内皮细胞、平滑肌细胞和成纤维细胞单层用终浓度为10⁻⁴至10⁻⁹M的尼古丁处理。将该效应记录为细胞骨架合成和聚合的变化。银染和金染方法在生理条件下产生了合成颗粒以及微管和细丝最终聚合物的各向异性，这通过偏光显微镜得以揭示。由于培养物中细胞的取向不均匀，取向的组织通过连接到OPTON细胞光度计的计算机化微光栅形态测量系统以交替对角线和正交各向异性测量的总和来表示。对8天大的对照和处理培养物也进行了电子显微镜检查。尼古丁刺激了细胞骨架蛋白的合成和聚合。这种现象在平滑肌细胞中很明显，在内皮细胞中也部分存在。成纤维细胞不受所测试尼古丁剂量的影响。
