Massaut Khadija Bezerra, Vitola Helena Reissing Soares, Gonçalves Vitória Sequeira, Leite Fabio Pereira Leivas, Jardim Rodrigo Desessards, Moreira Ângela Nunes, da Silva Wladimir Padilha, Fiorentini Ângela Maria
Laboratory of Food Microbiology, Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas, RS, Brazil.
Biotechnology Unit, Technology Development Center, Federal University of Pelotas, Pelotas, RS, Brazil.
Probiotics Antimicrob Proteins. 2024 Sep 24. doi: 10.1007/s12602-024-10362-9.
The study aimed to evaluate the effects of supplementation with Lacticaseibacillus casei CSL3 in Swiss mice immunosuppressed with cyclophosphamide on immunological, biochemical, oxidative stress, and histological parameters. The animals were distributed into four groups (control, CSL3, cyclophosphamide, and CSL3 + cyclophosphamide), where two groups were treated with L. casei CSL3 (10 log CFU mL) for 30 days, and two groups received chemotherapy (days 27 and 30-total dose of 250 mg kg). Counts of lactic acid bacteria (LAB) and bile-resistant LAB in stool samples; blood count (erythrogram, leukogram, and platelets); serum total cholesterol levels; catalase enzyme activity; and thiobarbituric acid reactive substances (TBARS) levels in liver, kidney, and brain; IL-4 expression; IL-23, TNF-α, NF-κβ in the spleen; and histological changes in the liver, kidneys, and intestine were evaluated. The CSL3 + cyclophosphamide group showed a significant increase in bile-resistant LAB counts in feces (p = 0.0001), leukocyte counts, and expression of IL-23, TNF-α, and NF-κβ (p < 0.05) significantly reduced total cholesterol levels (p = 0.001) and protected liver damage of supplemented animals. For oxidative stress damage, the bacterium did not influence the results. It is concluded that the bacterium is safe at a concentration of 10 log CFU mL and has probiotic potential due to its positive influence on the immune response and lipid metabolism.
本研究旨在评估用干酪乳杆菌CSL3补充环磷酰胺免疫抑制的瑞士小鼠后，对其免疫、生化、氧化应激和组织学参数的影响。将动物分为四组（对照组、CSL3组、环磷酰胺组和CSL3 +环磷酰胺组），其中两组用干酪乳杆菌CSL3（10 log CFU/mL）处理30天，两组接受化疗（第27天和第30天——总剂量250 mg/kg）。评估粪便样本中乳酸菌（LAB）和耐胆汁LAB的计数；血常规（红细胞计数、白细胞计数和血小板计数）；血清总胆固醇水平；过氧化氢酶活性；肝脏、肾脏和大脑中硫代巴比妥酸反应性物质（TBARS）水平；IL-4表达；脾脏中IL-23、TNF-α、NF-κβ；以及肝脏、肾脏和肠道的组织学变化。CSL3 +环磷酰胺组粪便中耐胆汁LAB计数显著增加（p = 0.0001），白细胞计数以及IL-23、TNF-α和NF-κβ的表达（p < 0.05）显著降低总胆固醇水平（p = 0.001），并保护补充动物的肝脏免受损伤。对于氧化应激损伤，该细菌不影响结果。得出的结论是，该细菌在浓度为10 log CFU/mL时是安全的，并且由于其对免疫反应和脂质代谢的积极影响而具有益生菌潜力。
