Saleh E A, Mahmoud S A, el-Haddad M E, Abdel-Fatah M K
Zentralbl Mikrobiol. 1985;140(4):325-32.
Complete extraction of S. aureofaciens lD13 antibiotic was achieved by adding n-butanol to the clarified culture filtrate (v/2v) at pH 8.0. Using the column chromatography technique, 85.7% of the initial amount of the antibiotic was obtained in a purified form. Data of the Rf values of the antibiotic in different organic solvents revealed that it belongs to the tetracycline group. The antibiotic was chromatographically analyzed, using the thin-layer technique. UV and IR spectra, optical rotation, melting point as well as 15 colour reactions were also determined.
在pH 8.0条件下，向澄清的培养滤液中加入正丁醇（体积比1:2），可实现对金色链霉菌lD13抗生素的完全提取。采用柱色谱技术，以纯化形式获得了初始量85.7%的抗生素。该抗生素在不同有机溶剂中的比移值数据表明它属于四环素类。采用薄层技术对该抗生素进行了色谱分析。还测定了紫外光谱、红外光谱、旋光度、熔点以及15种显色反应。
