Ahmad Islamudin, Febrina Lizma, Hikmawan Baso Didik, Junaidin Junaidin, Herman Herman, Faisal Muhammad, Samsul Erwin, Iswahyudi Iswahyudi, Ibrahim Arsyik, Purwoko Reza Yuridian, Okselni Tia, Angelina Marissa, Putra Amal Rezka, Alwi Ratna Surya, Mun'im Abdul
Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia.
Department of Research and Development, PT. Borneo Riseta Naturafarm, Kutai Kertanegara, Indonesia.
Nat Prod Res. 2024 Sep 25:1-8. doi: 10.1080/14786419.2024.2406996.
The metabolite profile of the ethanol extract from was assessed using the 1D NMR approach amalgamated with 2D NMR. an indigenous plant found in Indonesia, possesses considerable promise as a raw material for medicinal products. We examined the antioxidant capacity of the extract using DPPH and ABTS methods. The antioxidant evaluation demonstrated that the ethanol extract exhibits strong antioxidant properties. Furthermore, we have successfully identified thirteen metabolites in the ethanol extract without separation. These metabolites include eleutherol A, eleutherol B, eleutherol C, eleuthione B, eleuthione C, hongconin, karwinaphthol, isoeleuthoside C, quinic acid, chlorogenic acid, isoeleutherin, cyanidin-3-o-β-glucopyranoside, and kadsuric acid. Metabolite Cyanidin-3-o-β-glucopyranoside (4.89 mM ± 0.02) was the highest metabolite contained in this extract. This result showed that NMR methods could detect and measure metabolites in the ethanol extract without requiring separation.
采用一维核磁共振（1D NMR）方法结合二维核磁共振（2D NMR）对[某种植物名称未给出]乙醇提取物的代谢物谱进行了评估。[该植物名称未给出]是一种在印度尼西亚发现的本土植物，作为药品原料具有相当大的潜力。我们使用DPPH和ABTS方法检测了[该植物名称未给出]提取物的抗氧化能力。抗氧化评估表明，乙醇提取物具有很强的抗氧化特性。此外，我们成功地在未分离的情况下鉴定出乙醇提取物中的13种代谢物。这些代谢物包括刺五加醇A、刺五加醇B、刺五加醇C、刺五加硫醚B、刺五加硫醚C、红康宁、卡维萘酚、异刺五加苷C、奎宁酸、绿原酸、异刺五加素、矢车菊素 - 3 - o - β - 葡萄糖苷和南五味子酸。代谢物矢车菊素 - 3 - o - β - 葡萄糖苷（4.89 mM ± 0.02）是该提取物中含量最高的代谢物。这一结果表明，核磁共振方法无需分离即可检测和测量[该植物名称未给出]乙醇提取物中的代谢物。
