Graduated of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Department of Pathobiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Vet Med Sci. 2024 Nov;10(6):e1581. doi: 10.1002/vms3.1581.
Adult tendon tissue has limited and slow regenerative capacity. Sambucus nigra plant possesses antioxidant and anti-inflammatory attributes.
This study aimed to evaluate the effect of hydroalcoholic extract of this plant's fruit on superficial digital flexor tendon repair in rabbits (SDFT).
Twenty-five male New Zealand white rabbits weighing 1.5-2 kg were selected, quarantined and randomly divided into four groups of six. By performing a partial left posterior limb tenotomy, differentiating the SDFT and creating multiple scrapes were performed. During 3 consecutive days post-surgery, the positive control group was injected with 0.5 mg/kg dexamethasone, whereas the treatment groups received extract doses of 200 and 400 mg/kg, respectively. The negative control group did not receive any medication. Evaluation of sonographic and histopathological parameters was conducted on days 0, 7 and 28 post-surgeries. Findings were analysed and compared using SPSS22.
Both treatment groups showed significant differences in echogenicity, and collagen fibre alignment compared to the control and positive control groups, in sonographic evaluation. Histopathological examination revealed fewer inflammatory cells and increased collagen fibre formation in the treatment groups compared to the other two groups. No significant difference in angiogenesis was observed among the groups on days 7 and 28 (p value <0.05).
The results indicate that the S. nigra fruit extract, by stimulating collagen synthesis and reducing inflammation, effectively accelerates the healing process of injured tendons.
成人肌腱组织的再生能力有限且缓慢。黑接骨木植物具有抗氧化和抗炎特性。
本研究旨在评估该植物果实的水醇提取物对兔（SDFT）浅表指屈肌腱修复的影响。
选择 25 只 1.5-2kg 的雄性新西兰白兔，进行检疫并随机分为四组，每组 6 只。通过进行部分左后肢肌腱切开术，区分 SDFT 并进行多次划痕。在手术后连续 3 天内，阳性对照组注射 0.5mg/kg 地塞米松，而治疗组分别接受 200 和 400mg/kg 的提取物剂量。阴性对照组未接受任何药物治疗。在手术后 0、7 和 28 天进行超声和组织病理学参数评估。使用 SPSS22 分析和比较结果。
在超声评估中，与对照组和阳性对照组相比，两组治疗组的回声强度和胶原纤维排列均有显著差异。组织病理学检查显示，与其他两组相比，治疗组的炎症细胞较少，胶原纤维形成增加。在第 7 天和第 28 天，各组的血管生成无显著差异（p 值<0.05）。
结果表明，黑接骨木果实提取物通过刺激胶原合成和减少炎症，有效加速受损肌腱的愈合过程。
