Ferreira Sandrine S, Martins-Gomes Carlos, Nunes Fernando M, Silva Amélia M
Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5001-801 Vila Real, Portugal.
Chemistry Research Center - Vila Real (CQ-VR), Food and Wine Chemistry Lab., University of Trás-os-Montes and Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal.
Food Chem X. 2022 Aug 27;15:100437. doi: 10.1016/j.fochx.2022.100437. eCollection 2022 Oct 30.
Despite the high value of Portuguese elderberries, recognized for decades by European markets, only a few studies address their beneficial effects at cellular level. Aiming to explore the anti-inflammatory and the cellular antioxidant potential characterized extracts from the three main Portuguese elderberry cultivars (Sabugueiro, Sabugueira, Bastardeira) were used. Lipopolysaccharide-stimulated RAW 264.7 cells pre-exposed to elderberry extracts exhibited dose-dependent inhibition of nitric oxide release, evidencing anti-inflammatory activity. Concerning cellular antioxidant protection, HepG2 and Caco-2 cells pre-exposure to elderberry extracts (50 µg/mL) prevented up-to 90 % of -butyl hydroperoxide (-BOOH)-induced toxicity. In Caco-2 cells, elderberry extracts prevented glutathione depletion, reactive oxygen species production, abnormal morphological changes and DNA fragmentation, in response to -BOOH oxidative insult. Results demonstrated that elderberries have high potential in reducing cellular oxidative stress as well as in preventing inflammatory processes. Thus, elderberries have high potential as health promoters, acting as functional foods or as sources of nutraceuticals.
尽管葡萄牙接骨木果价值很高，几十年来一直为欧洲市场所认可，但只有少数研究探讨了其在细胞水平上的有益作用。为了探索抗炎和细胞抗氧化潜力，使用了来自葡萄牙三个主要接骨木果品种（Sabugueiro、Sabugueira、Bastardeira）的特征提取物。预先暴露于接骨木果提取物的脂多糖刺激的RAW 264.7细胞表现出一氧化氮释放的剂量依赖性抑制，证明了抗炎活性。关于细胞抗氧化保护，预先将HepG2和Caco-2细胞暴露于接骨木果提取物（50μg/mL）可预防高达90%的叔丁基过氧化氢（t-BOOH）诱导的毒性。在Caco-2细胞中，接骨木果提取物可预防谷胱甘肽耗竭、活性氧生成、异常形态变化和DNA片段化，以应对t-BOOH氧化损伤。结果表明，接骨木果在降低细胞氧化应激以及预防炎症过程方面具有很高的潜力。因此，接骨木果作为健康促进剂具有很高的潜力，可作为功能性食品或营养保健品的来源。
