Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
Prostaglandins Other Lipid Mediat. 2024 Dec;175:106911. doi: 10.1016/j.prostaglandins.2024.106911. Epub 2024 Sep 23.
It was aimed to determine the potential effect of Lactobacillus acidophilus supplementation on rats exposed to an experimental high-fat diet on serum lipid profile and kidney total beta amyloid protein (TBAP) and Tumor Necrosis Factor-alpha (TNF-α) levels.
24 male Sprague-Dawley rats were used in the study to establish 4 groups. Standard rat food (SD) was provided to Group 1 as the control; Group 2 was fed a high-fat diet (HFD); Group 3 consumed SD and received L. acidophilus probiotics; Group 4 was fed HFD and received L. acidophilus probiotics. Body weights were determined weekly during the 12-week trial period. At the end of the experiment, TBP and TNF-α levels in the serum and kidney tissue of the rats were measured by ELISA method. Serum total cholesterol (TC), triglyceride (TG), HDL, LDL, urea and creatinine levels and paraoxanase, amylase and lipase activities were determined by spectrophotometric method on the analyzer device.
When the control (Group 1) group and Group 2 were compared at the end of the experiment, it was found that Group 2 had gained the most weight and that both the blood and kidney tissue levels of TNF-α and TBAP, as well as the quantities of TG, TK, LDL, and urea, were significantly greater (P<0.05). Serum HDL, PON and amylase levels were found to be significantly low (P>0.05). TG, TK, LDL, urea, and the levels of TNF-α and TBAP in serum and renal tissue were shown to be lower in the groups who received L. acidophilus probiotics (Groups 3, 4) when compared to Group 2 (P>0.05). It was observed that HDL, PON and amylase levels increased and approached the control group (P<0.05).
The study's findings showed that probiotic supplementation improved blood levels of TG, TC, HDL, LDL, urea, PON, and amylase as well as serum and kidney tissue levels of TNF-α and TBAP in obese rats fed a high-fat diet.
旨在确定嗜酸乳杆菌补充剂对暴露于实验性高脂肪饮食的大鼠的血清脂质谱以及肾总β淀粉样蛋白（TBAP）和肿瘤坏死因子-α（TNF-α）水平的潜在影响。
本研究使用 24 只雄性斯普拉格-道利大鼠建立 4 组。第 1 组给予标准大鼠食物（SD）作为对照；第 2 组给予高脂肪饮食（HFD）；第 3 组给予 SD 并接受嗜酸乳杆菌益生菌；第 4 组给予 HFD 并接受嗜酸乳杆菌益生菌。在 12 周的试验期间，每周测量体重。实验结束时，通过 ELISA 法测量大鼠血清和肾组织中 TBAP 和 TNF-α的水平。通过分光光度法在分析仪设备上测定血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、尿素和肌酐水平以及对氧磷酶、淀粉酶和脂肪酶的活性。
在实验结束时，将对照组（第 1 组）与第 2 组进行比较，发现第 2 组体重增加最多，并且 TNF-α和 TBAP 的血液和肾组织水平以及 TG、TK、LDL 和尿素的量均显著增加（P<0.05）。血清 HDL、PON 和淀粉酶水平明显较低（P>0.05）。与第 2 组相比，接受嗜酸乳杆菌益生菌的组（第 3 组、第 4 组）的 TG、TK、LDL、尿素以及血清和肾组织中 TNF-α和 TBAP 的水平较低（P>0.05）。观察到 HDL、PON 和淀粉酶水平增加并接近对照组（P<0.05）。
研究结果表明，益生菌补充剂改善了高脂肪饮食喂养肥胖大鼠的血液 TG、TC、HDL、LDL、尿素、PON 和淀粉酶水平以及血清和肾组织中 TNF-α和 TBAP 的水平。
