Çayli Berna, Çiftci Gülay, Çiftci Alper, Savaşan Sadık
Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
Prostaglandins Other Lipid Mediat. 2023 Jun;166:106729. doi: 10.1016/j.prostaglandins.2023.106729. Epub 2023 Mar 11.
One of the rapidly rising global public health concern is obesity. Over the past three decades, the prevalence of obesity has doubled/tripled in several nations around the world, most likely as a result of urbanization, sedentary lifestyles, and increased intake of high-calorie processed foods. In this study, it was aimed to investigate the effects of Lactobacillus acidophilus administration on rats exposed to high-fat diet experimentally on anorexigenic peptides in the brain and some biochemical parameters in the serum.
In the study, 4 different experimental groups were formed. Group 1 was designated as the control group and fed with a standard rat chow (SD). Group 2 was designated as the high-fat diet (HFD) fed group. Group 3 fed with SD and L. acidophilus probiotic administered. Group 4 fed with HFD and L. acidophilus probiotic administered. At the end of the experiment, leptin, serotonin, glucagon-like peptide-1 (GLP-1) levels were measured in the brain tissue and serum. Glucose, total cholesterol (TC), triglyceride (TG), total protein (TP), albumin, uric acid, aspartate transaminase (AST), alanine aminotransferase (ALT) levels were determined in the serum.
At the end of the study, it was found that there was an increase in body weight and body mass index in Group 2 compared to Group 1. It was determined that the levels of AST, ALT, TG, TC, glucose, leptin in the serum were significantly high (P < 0.05). The levels of GLP-1 and serotonin in the serum and in the brain were significantly low (P < 0.05). There was a significant decrease in TG and TC in Groups 3 and 4 compared to Group 2 (P < 0.05). The leptin hormone levels in serum and brain were significantly higher in Group 2 than in other groups (P < 0.05). GLP-1 and serotonin levels were found to be significantly low (P < 0.05). The leptin levels in the serum of Groups 3 and 4 decreased significantly compared to Group 2 (P < 0.05).
It was found that probiotic supplementation in high-fat diet had positive effects on anorexigenic peptides. It was concluded that L. acidophilus probiotic can be recommended as a food supplement in the treatment of obesity.
肥胖是全球迅速增长的公共卫生问题之一。在过去三十年里，全球多个国家的肥胖患病率翻了一番/两番，这很可能是城市化、久坐不动的生活方式以及高热量加工食品摄入量增加所致。本研究旨在探讨给予嗜酸乳杆菌对实验性高脂饮食大鼠大脑中厌食肽及血清中一些生化参数的影响。
本研究组建了4个不同的实验组。第1组为对照组，喂食标准大鼠饲料（SD）。第2组为高脂饮食（HFD）喂养组。第3组喂食SD并给予嗜酸乳杆菌益生菌。第4组喂食HFD并给予嗜酸乳杆菌益生菌。实验结束时，测量脑组织和血清中的瘦素、血清素、胰高血糖素样肽-1（GLP-1）水平。测定血清中的葡萄糖、总胆固醇（TC）、甘油三酯（TG）、总蛋白（TP）、白蛋白、尿酸、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）水平。
研究结束时，发现第2组的体重和体重指数相比第1组有所增加。血清中AST、ALT、TG、TC、葡萄糖、瘦素水平显著升高（P<0.05）。血清和大脑中的GLP-1和血清素水平显著降低（P<0.05）。与第2组相比，第3组和第4组的TG和TC显著降低（P<0.05）。第2组血清和大脑中的瘦素激素水平显著高于其他组（P<0.05）。GLP-1和血清素水平显著降低（P<0.05）。第3组和第4组血清中的瘦素水平相比第2组显著降低（P<0.05）。
发现高脂饮食中补充益生菌对厌食肽具有积极作用。得出结论，嗜酸乳杆菌益生菌可作为肥胖治疗中的一种食品补充剂被推荐。
