Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Research Center for Environmental Contaminants (RCEC), Abadan University of Medical Sciences, Abadan, Iran.
Clin Chim Acta. 2025 Jan 15;565:119976. doi: 10.1016/j.cca.2024.119976. Epub 2024 Sep 24.
Thrombin, a key factor in the coagulation cascade, is a valuable biomarker of great importance for the prognosis, diagnosis, and monitoring of various diseases, including cancer and heart disease. Due to the increasing attention to the development of point-of-care testing (POCT) options, various types of biosensors have been invented to enhance the accuracy and speed of detection of important biomarkers such as thrombin. Implementation of aptamers in biosensors (aptasensors) improves the target recognition capacity due to the high-affinity binding nature of aptamers. Herein, this review presents recent studies of aptasensors for thrombin detection based on different detection mechanisms encompassing optical biosensors, surface-enhanced Raman spectroscopy (SERS), electrochemical detection, piezoelectric detection, and lateral flow assay.
凝血酶是凝血级联反应中的关键因素，是各种疾病（包括癌症和心脏病）的预后、诊断和监测的重要生物标志物。由于人们越来越关注即时检测（POCT）选项的开发，因此发明了各种类型的生物传感器，以提高对重要生物标志物（如凝血酶）的检测准确性和速度。适体在生物传感器（适体传感器）中的应用提高了目标识别能力，因为适体具有高亲和力结合的性质。本文综述了基于不同检测机制的基于适体的凝血酶检测生物传感器的最新研究，这些检测机制包括光学生物传感器、表面增强拉曼光谱（SERS）、电化学检测、压电检测和侧向流动分析。
