Pincus S H, Cammarata P
Immunology. 1985 Oct;56(2):219-25.
Eosinophils play an important role in host defence against helminthic infection. A quantitative system for studying the interactions of eosinophils and a large non-ingestible target was developed using infective muscle-stage larvae of Trichinella spiralis. At 37 degrees, this antibody-dependent system provided a reliable, reproducible index of eosinophil function. Adherence was equivalent under aerobic and anaerobic conditions. No significant differences were noted between peritoneal exudate eosinophils from Trichinella-infected and normal animals. Adherence was inhibited by leukotriene inhibitors [eicosaetraynoic acid (10(-3) M), nordihydroguaiaretic acid (10(-4) M), and BW755C (10(-3) M)] but not by prostaglandin inhibitors (aspirin and indomethacin), suggesting that leukotrienes mediate this eosinophil function. This assay provides another useful method for assessment of a crucial eosinophil function.
嗜酸性粒细胞在宿主抵御蠕虫感染中发挥着重要作用。利用旋毛虫感染性肌幼虫，开发了一种用于研究嗜酸性粒细胞与大型不可吞噬靶标相互作用的定量系统。在37摄氏度时，这个抗体依赖系统提供了一个可靠、可重复的嗜酸性粒细胞功能指标。在有氧和无氧条件下，嗜酸性粒细胞的黏附情况相同。来自旋毛虫感染动物和正常动物的腹腔渗出嗜酸性粒细胞之间未观察到显著差异。白三烯抑制剂[二十碳五烯酸（10⁻³ M）、去甲二氢愈创木酸（10⁻⁴ M）和BW755C（10⁻³ M）]可抑制嗜酸性粒细胞的黏附，但前列腺素抑制剂（阿司匹林和吲哚美辛）则不能，这表明白三烯介导了这种嗜酸性粒细胞功能。该检测方法为评估一项关键的嗜酸性粒细胞功能提供了另一种有用的方法。
