Pincus S H
Cell Immunol. 1984 Jul;86(2):460-71. doi: 10.1016/0008-8749(84)90401-5.
In its role as an effector capable of killing large multicellular parasites, the eosinophil must be especially adapted for dealing with noningestible surfaces. A model system of Sepharose beads coated with serum protein or concanavalin A (Con A) has been used to study interactions between guinea pig peritoneal exudate eosinophils and noningestible particles. A small percentage of eosinophils were adherent to serum treated Sepharose; however, many cells were adherent to Con A-Sepharose. Adherence to Con A-Sepharose was decreased by pretreatment with 1 mM alpha-methylmannoside (alpha-MM). As compared to resting eosinophils, incubation of eosinophils with serum-treated Sepharose led to activation of oxidative metabolism as indicated by an eight-fold increase in superoxide anion production and an approximately threefold increase in quantitative leukocyte iodination. Eosinophils which were adherent to Con A beads could not be activated by either phorbol myristate acetate (PMA) or preopsonized zymosan. However, if adherence was reduced by preincubation with alpha-MM, PMA was able to activate the eosinophils. Neither soluble Con A nor Sepharose beads interfered with the assay of superoxide anion. These studies demonstrate the utility of Sepharose beads for studying interactions between eosinophils and noningestible particles.
作为一种能够杀死大型多细胞寄生虫的效应细胞，嗜酸性粒细胞必须特别适应处理不可吞噬的表面。已使用涂有血清蛋白或伴刀豆球蛋白A（Con A）的琼脂糖珠模型系统来研究豚鼠腹腔渗出嗜酸性粒细胞与不可吞噬颗粒之间的相互作用。一小部分嗜酸性粒细胞附着于经血清处理的琼脂糖；然而，许多细胞附着于Con A-琼脂糖。用1 mM α-甲基甘露糖苷（α-MM）预处理可降低对Con A-琼脂糖的附着。与静息嗜酸性粒细胞相比，用经血清处理的琼脂糖孵育嗜酸性粒细胞会导致氧化代谢激活，超氧阴离子产生增加八倍以及定量白细胞碘化增加约三倍表明了这一点。附着于Con A珠的嗜酸性粒细胞不能被佛波酯肉豆蔻酸酯乙酸盐（PMA）或调理过的酵母聚糖激活。然而，如果通过与α-MM预孵育降低附着，PMA能够激活嗜酸性粒细胞。可溶性Con A和琼脂糖珠均不干扰超氧阴离子的测定。这些研究证明了琼脂糖珠在研究嗜酸性粒细胞与不可吞噬颗粒之间相互作用方面的实用性。
