Sartorelli L, Ciman M, Siliprandi N
Ital J Biochem. 1985 Jul-Aug;34(4):275-81.
Rat heart slices show a permeability barrier that can be crossed by carnitine but not by sucrose and inulin. The integrity of thiol groups of heart cell membrane is essential for the uptake of carnitine. N-ethylmaleimide inhibits the transport into heart slices which is insensitive to Mersalyl. On the contrary both N-ethylmaleimide and Mersalyl inhibit acetyl carnitine/carnitine exchange. The amount of thiol groups titrated by the above reagents are related to the extent of exchange inhibition.
大鼠心脏切片显示存在一种通透性屏障，肉碱可以穿过，但蔗糖和菊粉则不能。心脏细胞膜硫醇基团的完整性对于肉碱的摄取至关重要。N-乙基马来酰胺抑制肉碱向心脏切片中的转运，而这种转运对汞撒利不敏感。相反，N-乙基马来酰胺和汞撒利都抑制乙酰肉碱/肉碱的交换。上述试剂滴定的硫醇基团数量与交换抑制程度相关。
