Ordal G W, Parker H M, Kirby J R
J Bacteriol. 1985 Nov;164(2):802-10. doi: 10.1128/jb.164.2.802-810.1985.
A set of chemotaxis mutants of Bacillus subtilis was complemented by using SP beta c2 transducing bacteriophage either containing cloned segments of DNA or derived from abnormal excision of SP beta c2 dl2::Tn917 inserted into the chemotaxis region. Representative mutants were characterized in capillary assays for chemotaxis toward four amino acids and mannitol and in tethered-cell experiments for addition and removal of two attractants and two repellents. Twenty complementation groups were identified, in addition to the cheR previously characterized. All were found to be defective in chemotaxis toward all chemoeffectors. They were assigned the names cheA through cheU. The large number of general chemotaxis genes in B. subtilis, in contrast to the six in Escherichia coli, suggests fundamental differences in the mechanism of chemotaxis in the two species.
利用SPβc2转导噬菌体对一组枯草芽孢杆菌趋化性突变体进行了互补实验，该噬菌体要么含有克隆的DNA片段，要么来源于插入趋化性区域的SPβc2 dl2::Tn917的异常切除。在毛细管实验中，对向四种氨基酸和甘露醇的趋化性进行了表征，在拴系细胞实验中，对添加和去除两种引诱剂和两种驱避剂进行了表征。除了先前表征的cheR外，还鉴定出20个互补组。发现所有这些在对所有化学效应物的趋化性方面都存在缺陷。它们被命名为cheA至cheU。与大肠杆菌中的六个趋化性基因相比，枯草芽孢杆菌中大量的一般趋化性基因表明这两个物种在趋化性机制上存在根本差异。
