Goldman D J, Worobec S W, Siegel R B, Hecker R V, Ordal G W
Biochemistry. 1982 Mar 2;21(5):915-20. doi: 10.1021/bi00534a016.
By performing in vivo methylation experiments and using highly resolving NaDodSO4-polyacrylamide gels, we have examined the effects of amino acid attractants on the methylation profile of Bacillus subtilis MCPs. Both increases and decreases have been found to occur in the level of methylation of these proteins. By using competition experiments and Conway diffusion cells, we have found that the demethylation event is correlated with the adaptation process. Gas chromatographic analysis indicates that methanol is evolved upon demethylation of these proteins. As more attractant receptors are titrated, corresponding increases in methanol evolution result. During this period of increased rate of methanol production, bacteria swim smoothly.
通过进行体内甲基化实验并使用高分辨率的十二烷基硫酸钠 - 聚丙烯酰胺凝胶，我们研究了氨基酸引诱剂对枯草芽孢杆菌甲基化趋化蛋白（MCPs）甲基化图谱的影响。已发现这些蛋白质的甲基化水平既有增加也有降低。通过竞争实验和康威扩散池，我们发现去甲基化事件与适应过程相关。气相色谱分析表明，这些蛋白质去甲基化时会释放出甲醇。随着更多的引诱剂受体被滴定，甲醇释放量相应增加。在甲醇产生速率增加的这段时间里，细菌游动顺畅。
