Tanaka K, Welsh F A, Greenberg J H, O'Flynn R, Harris V A, Reivich M
J Cereb Blood Flow Metab. 1985 Dec;5(4):502-11. doi: 10.1038/jcbfm.1985.76.
Local CMRgl (LCMRgl) and metabolite levels were measured in the same tissue samples following 4 h of recirculation after 1 h of occlusion of the middle cerebral artery in the cat. The rate of glucose utilization was calculated using direct measurement of tissue deoxyglucose-6-phosphate and using a "lumped" constant corrected in each sample for alterations in tissue glucose. Increased LCMRgl (compared with that in sham-operated animals) occurred in regions with only minor alterations in levels of lactate and phosphocreatine. By contrast, LCMRgl was markedly depressed in regions with major changes in lactate and high-energy phosphates. Interestingly, tissue levels of glucose and unphosphorylated deoxyglucose were abnormally elevated in regions with profound energy failure. These results indicate an inhibition of glucose utilization in regions damaged by ischemia, despite the persistent elevation of tissue lactate. Increased glucose metabolism at 4 h post ischemia was detected only in areas with minor anaerobic alteration of metabolite levels.
在猫大脑中动脉闭塞1小时后再灌注4小时，对相同组织样本测量局部脑代谢率葡萄糖（LCMRgl）和代谢物水平。利用组织6 - 磷酸脱氧葡萄糖的直接测量值，并使用针对每个样本中组织葡萄糖变化校正的“集总”常数来计算葡萄糖利用率。在乳酸和磷酸肌酸水平仅有轻微变化的区域出现了LCMRgl升高（与假手术动物相比）。相比之下，在乳酸和高能磷酸盐发生重大变化的区域，LCMRgl显著降低。有趣的是，在能量严重衰竭的区域，葡萄糖和未磷酸化的脱氧葡萄糖的组织水平异常升高。这些结果表明，尽管组织乳酸持续升高，但在缺血受损区域葡萄糖利用受到抑制。仅在代谢物水平厌氧变化较小的区域检测到缺血后4小时葡萄糖代谢增加。
