Laboratory of Nutrition and Cancer, Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, 13083-970, Brazil.
Pharmaceutical Science Faculty, University of Campinas (UNICAMP), Campinas, 6109, São Paulo, 13083-970, Brazil.
Mol Nutr Food Res. 2024 Oct;68(20):e2400378. doi: 10.1002/mnfr.202400378. Epub 2024 Sep 27.
The combination of honey and Aloe vera is used as a popular complementary treatment for cancer due to their nutraceutical properties. This study aims to investigate the anticancer activity of honey and A. vera solution and its ethanolic extraction through in vitro and in vivo approaches.
After comparisons of honey and A. vera (HA) solution and its ethanolic extraction solution (E) samples by UPLC-ESI-MS/MS, the study verifies HA-treatment affected only Walker tumor cells viability at the highest dose, and E-treatment has a more cytotoxic/antiproliferative effect in MCF-7 and Walker-256 cells. The in vivo results show a higher survival rate in Walker-256 tumor-bearing rats (WHA), with higher NK cell infiltration in tumor tissue and a tendency in the WE group. These results are possible due to decreased mannose-based immunomodulatory polysaccharides and aloin-A contents in the ethanolic extract solution compared to HA solution.
The current study provides compelling evidence of selectively cytotoxic against tumor cells under honey and A. vera solution and ethanolic extraction solution treatment, due to the cytotoxic/antiproliferative compounds. Therefore, the use of honey and A. vera solution could be used as a basis for coadjuvant therapy in cancer treatment.
由于蜂蜜和芦荟具有营养特性，因此将其组合使用作为癌症的流行补充疗法。本研究旨在通过体外和体内方法研究蜂蜜和芦荟溶液及其乙醇提取物的抗癌活性。
通过 UPLC-ESI-MS/MS 对蜂蜜和芦荟（HA）溶液及其乙醇提取物（E）样品进行比较后，研究证实 HA 处理仅在最高剂量下影响 Walker 肿瘤细胞的活力，而 E 处理对 MCF-7 和 Walker-256 细胞具有更强的细胞毒性/抗增殖作用。体内结果显示，在 Walker-256 荷瘤大鼠（WHA）中，生存率更高，肿瘤组织中 NK 细胞浸润更多，WE 组有这种趋势。与 HA 溶液相比，乙醇提取物溶液中基于甘露糖的免疫调节多糖和芦荟素 A 的含量降低，这可能导致了这些结果。
本研究为蜂蜜和芦荟溶液以及乙醇提取物处理下选择性杀伤肿瘤细胞提供了令人信服的证据，这归因于细胞毒性/抗增殖化合物。因此，蜂蜜和芦荟溶液的使用可以作为癌症治疗辅助治疗的基础。
