Limsakul Suphatson, Monthakantirat Orawan, Chulikhit Yaowared, Maneenet Juthamart, Khamphukdee Charinya, Chotritthirong Yutthana, Phasomsap Achiraya, Boonyarat Chantana, Daodee Supawadee
Division of Pharmaceutical Chemistry Faculty of Pharmaceutical Sciences Khon Kaen University, Khon Kaen 40002, Thailand.
Natural Drug Discovery Laboratory Institute of Natural Medicine University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Adv Pharmacol Pharm Sci. 2024 Sep 18;2024:8335536. doi: 10.1155/2024/8335536. eCollection 2024.
The Trikaysornmas formula (TKM) represents a prevalent Thai traditional remedy utilized extensively in Thailand. Its traditional uses include appetite enhancement, functions as a nourishing tonic, and exhibits adaptogenic properties. Comprising fruit, stamen, and bark, this formula embodies the synergy among these three herbs. The objective of this study was to optimize the extraction method, determine the active compounds in the TKM, and evaluate its antioxidant activity. The optimization of the extraction method for this formula was studied using an experimental design. Phytochemical components such as total phenolics, total flavonoids, total carotenoids, and total alkaloids were assessed utilizing a colorimetric method. Antioxidant activities were assessed through DPPH free radical scavenging, ABTS radical cation decolorization, oxygen radical absorbance capacity, ferric reducing antioxidant power, metal chelating activity, and lipid peroxidation assay. For the analysis of active constituents in the formula, gallic acid, kaempferol-3-o-glucoside, imperatorin, vitexin, and scopoletin, a validated reversed-phase column high-performance liquid chromatography (HPLC) method was developed. The total active contents including phenolic, flavonoid, carotenoid, and alkaloid compounds were found in the formula. The developed HPLC method exhibited reliable results in all validation parameters. TKM demonstrated antioxidant activity in the models used in this research. The findings from this study can serve as valuable tools for standardization and quality control measures. Additionally, they can contribute to maximizing the possibilities inherent in this traditional Thai formulation.
特里卡约恩马斯配方（TKM）是一种在泰国广泛使用的流行泰国传统药物。它的传统用途包括增强食欲、作为滋补品以及具有适应原特性。该配方由果实、雄蕊和树皮组成，体现了这三种草药之间的协同作用。本研究的目的是优化提取方法，确定TKM中的活性化合物，并评估其抗氧化活性。使用实验设计研究了该配方提取方法的优化。采用比色法评估了总酚、总黄酮、总类胡萝卜素和总生物碱等植物化学成分。通过DPPH自由基清除、ABTS自由基阳离子脱色、氧自由基吸收能力、铁还原抗氧化能力、金属螯合活性和脂质过氧化测定来评估抗氧化活性。为了分析配方中的活性成分，开发了一种经过验证的反相柱高效液相色谱（HPLC）方法，用于分析没食子酸、山奈酚-3-o-葡萄糖苷、欧前胡素、牡荆素和东莨菪素。该配方中发现了包括酚类、黄酮类、类胡萝卜素和生物碱化合物在内的总活性成分。所开发的HPLC方法在所有验证参数中均显示出可靠的结果。TKM在本研究使用的模型中表现出抗氧化活性。本研究的结果可作为标准化和质量控制措施的宝贵工具。此外，它们有助于最大限度地发挥这种传统泰国配方的内在潜力。
