次氯酸钠、氯己定、蜂胶和氢氧化钙对脂磷壁酸诱导的促炎细胞因子产生影响的体外评估
In Vitro Evaluation of Sodium Hypochlorite, Chlorhexidine, Propolis, and Calcium Hydroxide Effect on Lipoteichoic-Acid-Induced Proinflammatory Cytokines Production.
作者信息
de Oliveira Luciane Dias, de Carvalho Lara Steffany, Xavier Ana Claudia Carvalho, de Oliveira Felipe Eduardo, Leão Mariella Vieira Pereira, Diamantino Mariana Gadelho Gimenez, Khoury Rayana Duarte, Valera Marcia Carneiro, Carvalho Cláudio Antonio Talge, Abu Hasna Amjad
机构信息
Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Campus of São José dos Campos, São Paulo State University, São Paulo 12245-000, Brazil.
Department of Restorative Dentistry, Endodontics Division, Institute of Science and Technology, Campus of São José dos Campos, São Paulo State University, São Paulo 12245-000, Brazil.
出版信息
Dent J (Basel). 2024 Sep 5;12(9):286. doi: 10.3390/dj12090286.
This study aimed to evaluate the effects of sodium hypochlorite (NaOCl), chlorhexidine (CHX), and the glycolic extract of propolis (GEP) as endodontic irrigants and of calcium hydroxide [Ca(OH)], CHX, or Ca(OH) + CHX as intracanal medications on the capacity of the lipoteichoic acid (LTA) of in macrophages' proinflammatory cytokines production. Freshly extracted 108 human single-rooted teeth were used in this study. The LTA of was standardized in double-distilled pyrogen-free water (250 µg/mL) and inoculated into the specimens subdivided into nine subgroups (n = 12). Cultures of murine macrophages (RAW 264.7) were treated with 30 µL of each sample collected from root canals and incubated (37 °C, 5% CO) for 24 h. Lastly, anti-TNF-α, anti-IL-6, anti-IP-10, anti-MIP-1α, anti-G-CSF, and anti-IL-1β DuoSet kits were used to perform an ELISA assay. Data were analyzed using one-way ANOVA and Tukey test ( ≥ 0.05). It was found that 1% NaOCl was the most effective irrigant in reducing the capacity of LTA in cytokines production, followed by 12% GEP and 2% CHX, respectively. Ca(OH) + CHX presented the best results when associated with NaOCl or GEP. Thus, NaOCl or GEP associated with Ca(OH) + CHX were effective in reducing the capacity of LTA in different macrophages pro-inflammatory cytokines production.
本研究旨在评估次氯酸钠（NaOCl）、氯己定（CHX）和蜂胶乙醇提取物（GEP）作为根管冲洗剂，以及氢氧化钙[Ca(OH)]、CHX或Ca(OH)+CHX作为根管内药物，对巨噬细胞中脂磷壁酸（LTA）促炎细胞因子产生能力的影响。本研究使用了108颗新鲜拔除的人单根牙。将的LTA在双蒸无热原水中标准化（250μg/mL），并接种到分为九个亚组（n=12）的标本中。用从根管收集的30μL每个样品处理小鼠巨噬细胞（RAW 264.7）培养物，并在（37℃，5%CO）下孵育24小时。最后，使用抗TNF-α、抗IL-6、抗IP-10、抗MIP-1α、抗G-CSF和抗IL-1β DuoSet试剂盒进行ELISA检测。数据采用单因素方差分析和Tukey检验进行分析（≥0.05）。结果发现，1%NaOCl是降低LTA细胞因子产生能力最有效的冲洗剂，其次分别是12%GEP和2%CHX。当与NaOCl或GEP联合使用时，Ca(OH)+CHX表现出最佳效果。因此，与Ca(OH)+CHX联合使用的NaOCl或GEP可有效降低不同巨噬细胞中LTA促炎细胞因子的产生能力。
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