Zhu Zihan, Zhang Yaqi, Wang Lei, Geng Haoyuan, Li Min, Chen Shiping, Wang Xiao, Chen Panpan, Sun Chenglong, Zhang Chao
Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Metabolites. 2024 Sep 2;14(9):482. doi: 10.3390/metabo14090482.
Curcumin is widely recognized for its diverse antitumor properties, ranging from breast cancer to many other types of cancers. However, its role in the tumor microenvironment remains to be elucidated. In this study, we established a 3D tumor spheroids model that can simulate the growth environment of tumor cells and visualized the antitumor metabolic alteration caused by curcumin using mass spectrometry imaging technology. Our results showed that curcumin not only exerts a profound impact on the growth and proliferation of breast cancer cells but in situ multivariate statistical analysis also reveals the significant effect on the overall metabolic profile of tumor spheroids. Meanwhile, our visualization map characterized curcumin metabolic processes of reduction and glucuronidation in tumor spheroids. More importantly, abnormal metabolic pathways related to lipid metabolism and polyamine metabolism were also remodeled at the metabolite and gene levels after curcumin intervention. These insights deepen our comprehension of the regulatory mechanism of curcumin on the tumor metabolic network, furnishing powerful references for antitumor treatment.
姜黄素因其多样的抗肿瘤特性而被广泛认可，其抗肿瘤范围涵盖从乳腺癌到许多其他类型的癌症。然而，其在肿瘤微环境中的作用仍有待阐明。在本研究中，我们建立了一种三维肿瘤球体模型，该模型可以模拟肿瘤细胞的生长环境，并使用质谱成像技术可视化姜黄素引起的抗肿瘤代谢改变。我们的结果表明，姜黄素不仅对乳腺癌细胞的生长和增殖产生深远影响，而且原位多变量统计分析还揭示了其对肿瘤球体整体代谢谱的显著影响。同时，我们的可视化图谱表征了姜黄素在肿瘤球体中的还原和葡萄糖醛酸化代谢过程。更重要的是，在姜黄素干预后，与脂质代谢和多胺代谢相关的异常代谢途径在代谢物和基因水平上也得到了重塑。这些见解加深了我们对姜黄素对肿瘤代谢网络调控机制的理解，为抗肿瘤治疗提供了有力的参考。
