Wu Shuang, Wang Yunhan, Han Shikai, Hui Ge, Teng Ye, Liu Wei, Zhao Yu
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 130017, China.
College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130017, China.
Mikrochim Acta. 2024 Sep 27;191(10):629. doi: 10.1007/s00604-024-06670-0.
A ratiometric fluorescence sensor (Fe-MIL-88-NH/curcumin) based on luminescent metal-organic frameworks (LMOFs) for the determination of curcumin was constructed. Upon the addition of curcumin, the 535-nm emission of curcumin was enhanced, while the fluorescence emission at 438 nm was quenched, under 367-nm excitation. This sensor demonstrated a broad linear range from 1.5 to 40 μM, a low detection limit of 35 nM, and a fast response time of at most 30 s. We verified the Förster resonance energy transfer (FRET) mechanism between donor (Fe-MIL-88-NH) and acceptor (curcumin), which further proved the selectivity of the approach. The sensing system enabled the detection of curcumin in the traditional Chinese medicine (TCM) Turmeric. A smartphone-assisted sensing platform was prepared to visually detect curcumin in a portable manner. This study represents the first attempt to fabricate LMOFs for ratiometric fluorescence detection of curcumin, which has promising potential for application in TCM.
构建了一种基于发光金属有机框架（LMOFs）的比率荧光传感器（Fe-MIL-88-NH/姜黄素）用于测定姜黄素。在加入姜黄素后，在367 nm激发下，姜黄素535 nm处的发射增强，而438 nm处的荧光发射猝灭。该传感器表现出1.5至40 μM的宽线性范围、35 nM的低检测限以及至多30 s的快速响应时间。我们验证了供体（Fe-MIL-88-NH）与受体（姜黄素）之间的福斯特共振能量转移（FRET）机制，这进一步证明了该方法的选择性。该传感系统能够检测中药姜黄中的姜黄素。制备了一个智能手机辅助传感平台，以便携式方式目视检测姜黄素。本研究是首次尝试制备用于姜黄素比率荧光检测的LMOFs，在中药中具有广阔的应用潜力。
