Pharmaceutical School, Nanchang University, Nanchang 330006, PR China.
Pharmaceutical School, Nanchang University, Nanchang 330006, PR China; Jiangxi Key Laboratory of Clinical Pharmacokinetics, Nanchang 330031, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Nov 5;320:124610. doi: 10.1016/j.saa.2024.124610. Epub 2024 Jun 6.
Developing rapid tetracycline sensing system is of great significance to monitor the illegal addition to drugs and pollution to food and ecosystem. By loading covalent organic frameworks (COFs) with Eu, a new hybridized material (COF@Eu) was prepared for tetracycline determination. Based on the Schiff base reaction, the COFs were by synthesized through solvent evaporation in 30 min at room temperature. Thereafter, Eu was modified into COFs to develop the COF@Eu sensing platform by adsorption and coordination. In presence of tetracycline, tetracycline can displace water molecules and coordinate with Eu through the antenna effect. As a result, the red fluorescence of Eu was enhanced by tetracycline with green fluorescence of COF as a reference. The developed ratiometric fluorescence sensor exhibits a linear range of 0.1-20 μM for detecting tetracycline with a detection limit of 30 nM. Integrated with a smartphone, the rapid tetracycline detection can be realized in situ, which is potential for high-throughput screening of tetracycline contaminated samples. Furthermore, the COF@Eu fluorescence sensor has been successfully applied to the detection of tetracycline in traditional Chinese medicine compound preparation with satisfied recoveries. Therefore, a smartphone-assisted device was successfully developed based on Eu-functionalized COF, which is an attractive candidate for further applications of fluorescence sensing and visual detection.
开发快速四环素传感系统对于监测药物中的非法添加物以及食物和生态系统的污染具有重要意义。通过负载铕的共价有机框架（COFs），制备了一种新的杂化材料（COF@Eu）用于四环素的测定。基于希夫碱反应，通过在室温下 30 分钟的溶剂蒸发合成 COFs。此后，通过吸附和配位将 Eu 修饰到 COFs 上，开发 COF@Eu 传感平台。在存在四环素的情况下，四环素可以通过天线效应置换水分子并与 Eu 配位。结果，Eu 的红色荧光通过四环素增强，而 COF 的绿色荧光作为参考。所开发的比率荧光传感器对四环素的检测线性范围为 0.1-20 μM，检测限为 30 nM。结合智能手机，可实现四环素的现场快速检测，有望用于高通量筛选四环素污染样品。此外，COF@Eu 荧光传感器已成功应用于中药复方制剂中四环素的检测，回收率令人满意。因此，成功地基于铕功能化 COF 开发了一种智能手机辅助设备，这是荧光传感和可视化检测进一步应用的有吸引力的候选者。
